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PXD033825-1

PXD033825 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIdentification of the quantitative phosphoproteome in mitotic HeLa cells with or without G6PD inhibitors.
DescriptionHeLa cells were synchronized with a double thymidine block procedure. Briefly, the exponentially growing HeLa cells were maintained with 2 mM thymidine for 18 h, followed by a release of 9 h in fresh medium, and then cells were re-cultured in 2 mM thymidine for additional 15 h. After a release of 7.5 h in fresh medium, DMSO or G6PD inhibitor was added to the medium. 1 h later, the cells entered into M phase. Mitotic cells were harvested by mitotic shake-off. Then, the samples were subjected to LC-MS/MS analysis. Finally, a Kinase-Substrate Enrichment was performed, which could infer the changes of upstream kinase activity upon the treatment of G6PD inhibitors.
HostingRepositoryPRIDE
AnnounceDate2023-05-18
AnnouncementXMLSubmission_2023-05-17_23:56:22.253.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAoxingCheng
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListphosphorylated residue
InstrumentQ Exactive HF; Q Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-05-12 02:04:16ID requested
12023-05-17 23:56:22announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: G6PD,Mitosis,Phosphoproteome
Contact List
ZhenyeYang
contact affiliationUniversity of Science and Technology of China
contact emailzhenye@ustc.edu.cn
lab head
AoxingCheng
contact affiliationUniversity of Science and Technology of China
contact emailchengax@mail.ustc.edu.cn
dataset submitter
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Dataset FTP location
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