PXD033825-1

PXD033825 is an original dataset announced via ProteomeXchange.

Title	Identification of the quantitative phosphoproteome in mitotic HeLa cells with or without G6PD inhibitors.
Description	HeLa cells were synchronized with a double thymidine block procedure. Briefly, the exponentially growing HeLa cells were maintained with 2 mM thymidine for 18 h, followed by a release of 9 h in fresh medium, and then cells were re-cultured in 2 mM thymidine for additional 15 h. After a release of 7.5 h in fresh medium, DMSO or G6PD inhibitor was added to the medium. 1 h later, the cells entered into M phase. Mitotic cells were harvested by mitotic shake-off. Then, the samples were subjected to LC-MS/MS analysis. Finally, a Kinase-Substrate Enrichment was performed, which could infer the changes of upstream kinase activity upon the treatment of G6PD inhibitors.
HostingRepository	PRIDE
AnnounceDate	2023-05-18
AnnouncementXML	Submission_2023-05-17_23:56:22.253.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	AoxingCheng
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive HF; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-12 02:04:16	ID requested
⏵ 1	2023-05-17 23:56:22	announced

Dataset with its publication pending

submitter keyword: G6PD,Mitosis,Phosphoproteome

ZhenyeYang
contact affiliation	University of Science and Technology of China
contact email	zhenye@ustc.edu.cn
lab head
AoxingCheng
contact affiliation	University of Science and Technology of China
contact email	chengax@mail.ustc.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD033825
     1. Label: PRIDE project
     2. Name: Identification of the quantitative phosphoproteome in mitotic HeLa cells with or without G6PD inhibitors.