PXD033825 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Identification of the quantitative phosphoproteome in mitotic HeLa cells with or without G6PD inhibitors. |
Description | HeLa cells were synchronized with a double thymidine block procedure. Briefly, the exponentially growing HeLa cells were maintained with 2 mM thymidine for 18 h, followed by a release of 9 h in fresh medium, and then cells were re-cultured in 2 mM thymidine for additional 15 h. After a release of 7.5 h in fresh medium, DMSO or G6PD inhibitor was added to the medium. 1 h later, the cells entered into M phase. Mitotic cells were harvested by mitotic shake-off. Then, the samples were subjected to LC-MS/MS analysis. Finally, a Kinase-Substrate Enrichment was performed, which could infer the changes of upstream kinase activity upon the treatment of G6PD inhibitors. |
HostingRepository | PRIDE |
AnnounceDate | 2023-05-18 |
AnnouncementXML | Submission_2023-05-17_23:56:22.253.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | AoxingCheng |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue |
Instrument | Q Exactive HF; Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-05-12 02:04:16 | ID requested | |
⏵ 1 | 2023-05-17 23:56:22 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: G6PD,Mitosis,Phosphoproteome |
Contact List
ZhenyeYang |
contact affiliation | University of Science and Technology of China |
contact email | zhenye@ustc.edu.cn |
lab head | |
AoxingCheng |
contact affiliation | University of Science and Technology of China |
contact email | chengax@mail.ustc.edu.cn |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD033825
- Label: PRIDE project
- Name: Identification of the quantitative phosphoproteome in mitotic HeLa cells with or without G6PD inhibitors.