PXD033640-2

PXD033640 is an original dataset announced via ProteomeXchange.

Title	Plasmid manipulation of bacterial behaviour through translational regulatory crosstalk
Description	pQBR103 is a naturally occurring plasmid known to alter the behaviour of its host, Pseudomonas fluorescens. This plasmid encodes a predicted translational regulator, rsmQ which is a homologue of known global regulators within Pseudomonas. Within this study we compared Pseudomonas fluorescens SBW25 plasmid free cells with cells carrying either WT pQBR103 or pQBR103 lacking rsmQ. Using comparative quantitative proteomics and RNAseq we showed that the loss of rsmQ leads to widespread proteomic changes in the absence of changes in mRNA abundance, suggesting RsmQ is a translational regulator of its host chromosome.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:56:25.063.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gerhard Saalbach
SpeciesList	scientific name: Pseudomonas fluorescens (strain SBW25); NCBI TaxID: 216595; scientific name: Bacteria; NCBI TaxID: NCBITaxon:2;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-04 14:39:56	ID requested
1	2023-01-13 03:39:14	announced
⏵ 2	2023-11-14 08:56:25	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: TMT, RNAseq, translational regulator,Pseudomonas fluorescens SBW25, quantitative proteomics, plasmid

Jacob G Malone
contact affiliation	Dept. Molecular Microbiology John Innes Centre Norwich Research Park Norwich, UK
contact email	jacob.malone@jic.ac.uk
lab head
Gerhard Saalbach
contact affiliation	Biological Chemistry
contact email	gerhard.saalbach@jic.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD033640
     1. Label: PRIDE project
     2. Name: Plasmid manipulation of bacterial behaviour through translational regulatory crosstalk