PXD033588-1

PXD033588 is an original dataset announced via ProteomeXchange.

Title	Ochlerotatus triseriatus saliva proteome
Description	Over the last 20 years, the advances in sequencing technologies highlighted the unique composition of the salivary glands of blood-feeding arthropods. Further biochemical and structural data demonstrated that salivary proteins can disrupt host hemostasis, inflammation and immune response in addition to favor pathogen transmission. Previously, a Sanger-based sialome of the adult Ochlerotatus. triseriatus female salivary glands was published based on 731 ESTs. Here we revisited O. triseriatus salivary glands contents using an Illumina-sequencing approach of both male and female tissues. In the current data set we report 10,317 coding DNA sequences classified into several functional classes. The translated transcripts also served as reference database for the proteomic analysis of O. triseriatus female saliva, in which unique peptides of 101 proteins were found. Finally, comparison of male and female libraries allowed the identification of female-enriched transcripts that are potentially related to blood acquisition and virus transmission.
HostingRepository	PRIDE
AnnounceDate	2022-10-15
AnnouncementXML	Submission_2022-10-14_21:16:23.882.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	StephenLu
SpeciesList	scientific name: Ochlerotatus triseriatus; NCBI TaxID: 7162;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-04 09:14:53	ID requested
⏵ 1	2022-10-14 21:16:24	announced
2	2023-11-14 08:34:05	announced	2023-11-14: Updated project metadata.
3	2024-10-22 05:40:36	announced	2024-10-22: Updated project metadata.

Lu S, Martin-Martin I, Ribeiro JM, Calvo E, A deeper insight into the sialome of male and female Ochlerotatus triseriatus mosquitoes. Insect Biochem Mol Biol, 147():103800(2022) [pubmed]

submitter keyword: saliva, mosquito, label-free quantification, proteome

EricCalvo
contact affiliation	Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, Bethesda, MD, USA.
contact email	ecalvo@niaid.nih.gov
lab head
StephenLu
contact affiliation	NIH/NIAID
contact email	stephen.lu@nih.gov
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD033588
     1. Label: PRIDE project
     2. Name: Ochlerotatus triseriatus saliva proteome