PXD033583-1

PXD033583 is an original dataset announced via ProteomeXchange.

Title	RcgA and RcgR, two novel proteins involved in conjugative transfer of rhizobial plasmids
Description	Rhizobia are gram-negative bacteria able to establish a symbiotic interaction with leguminous plants. Due to their nitrogen fixing capacity, the study of these microorganisms has acquired great relevance for the agriculture. Rhizobia usually harbor many plasmids in their genome which can be transferred to other organisms by conjugation. Two main mechanisms of regulation of rhizobial plasmid transfer have been described: Quorum sensing (QS) and rctA/rctB system. Nevertheless, new genes and molecules that modulate conjugative transfer have been recently described, demonstrating that new actors can tightly regulate the process. In this work, by means of bioinformatics tools and molecular biology approaches, two hypothetical genes are identified as playing key roles in conjugative transfer. These genes are located between conjugative genes of plasmid pLPU83a from Rhizobium favelukesii LPU83, a plasmid that showed a conjugative transfer behavior depending on the genomic background. One of the two mentioned genes, rcgA, is essential for conjugation, while the other, rcgR, acts as an inhibitor of the process. In addition to introducing this new regulatory mechanism, we show evidence of the functions of these genes in different genomic backgrounds, and confirmed that homologous proteins from non-closely related organisms play the same function. These findings set up a cornerstone for a new molecular circuit of conjugative transfer of plasmids.
HostingRepository	PRIDE
AnnounceDate	2022-08-29
AnnouncementXML	Submission_2022-08-29_05:53:08.932.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gonzalo Torres Tejerizo
SpeciesList	scientific name: Rhizobium favelukesii; NCBI TaxID: 348824;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-04 09:10:32	ID requested
⏵ 1	2022-08-29 05:53:09	announced
2	2023-11-14 08:56:23	announced	2023-11-14: Updated project metadata.

10.1128/MBIO.01949-22;

submitter keyword: Rhizobia, plasmid, conjugation

Gonzalo Torres Tejerizo
contact affiliation	Instituto de Biotecnología y Biología Molecular Facultad de Ciencias Exactas. Universidad Nacional de La Plata. CCT La Plata CONICET Calle 49 y 115 1900. La Plata Argentina
contact email	gatt@biol.unlp.edu.ar
lab head
Gonzalo Torres Tejerizo
contact affiliation	Instituto de Biotecnología y Biología Molecular Facultad de Ciencias Exactas. Universidad Nacional de La Plata. CCT La Plata CONICET Calle 49 y 115 1900. La Plata
contact email	gatt@biol.unlp.edu.ar
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD033583

PRIDE project URI

• PRIDE
  1. PXD033583
     1. Label: PRIDE project
     2. Name: RcgA and RcgR, two novel proteins involved in conjugative transfer of rhizobial plasmids