PXD033571-1
PXD033571 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Shared and distinctive neighborhoods of emerin and LBR revealed by proximity labeling and quantitative proteomics |
| Description | Emerin and LBR are abundant transmembrane proteins of the nuclear envelope (NE) that are concentrated at the inner nuclear membrane (INM). Although both proteins interact with chromatin and nuclear lamins, they have distinctive biochemical and functional properties. Here we have deployed proximity labeling using the engineered biotin ligase TurboID (TbID) and quantitative proteomics to compare the neighborhoods of emerin and LBR in cultured mouse embryonic fibroblasts (MEFs). Our analysis revealed 232 high confidence proximity partners (HCPP) that interact selectively with emerin and/or LBR, 49 of which are shared by both. These included previously characterized NE-concentrated proteins, as well as a host of additional proteins not previously linked to emerin or LBR functions. Many of these are TM proteins of the ER and include two E3 ubiquitin ligases. Using the proximity ligation assay as an orthogonal approach, we validated the interactions described by proximity labeling for 11/13 proteins analyzed, supporting the robustness of our analysis. Overall, this work presents methodology that may be used for large-scale mapping of the landscape of the INM and reveals a group of new proteins with potential functional connections to emerin and LBR. |
| HostingRepository | MassIVE |
| AnnounceDate | 2022-06-28 |
| AnnouncementXML | Submission_2022-06-28_17:02:30.441.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Salvador Martínez de Bartolomé |
| SpeciesList | scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090; |
| ModificationList | Phospho; Dimethyl; Dimethyl:2H(4); Dimethyl:2H(6)13C(2); Carbamidomethyl; Oxidation; TMT6plex |
| Instrument | Orbitrap Fusion; Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2022-05-02 12:38:38 | ID requested | |
| ⏵ 1 | 2022-06-28 17:02:30 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: nuclear envelope, nuclear lamina, proximity labeling, emerin, LBR |
Contact List
| Larry Gerace | |
|---|---|
| contact affiliation | The Scripps Research Institute |
| contact email | lgerace@scripps.edu |
| lab head | |
| Salvador Martínez de Bartolomé | |
| contact affiliation | The Scripps Research Institute |
| contact email | salvador@scripps.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000089356/ |
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