PXD033568-2
PXD033568 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Establishment of a landscape of UPL5-ubiquitinated on multiple subcellular components of leaf senescence cell in Arabidopsis |
Description | Catabolism of macromolecules is a major event in senescent cells, especially involving proteolysis of organelles and abnormally aggregated proteins, the circulation of nutrients, and precise control of intracellular environmental balance. Proteasomes are distributed in the nucleus and cytoplasm; however, proteasome in organelles is limited. In this study, multi-omics proteomic analyses of ubiquitinated proteins enriched by using antibody against “di-Gly-Lys” via a free labeling was used to investigate the global changes of protein levels and ubiquitination modification levels of upl5 mutant relative to wildtype plant; subcellular localization analysis of UPL5 was found to be located in the nucleus, cytoplasm and plastid within the cell; and the direct lysine site pattern of UPL5 were screened by the H89R substitution in the tagged ubiquitinated assay. It suggests that UPL5 acting as a candidate of organelle E3 ligase either in the nucleus or cytoplasm or plastid, modifies numerous targets related to nuclear transcription and plastid photosynthesis involving in Ca2+ and hormone signaling pathway in plant senescence and in response to (a)biotic stress protection. |
HostingRepository | iProX |
AnnounceDate | 2022-04-30 |
AnnouncementXML | Submission_2023-05-06_00:10:25.330.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Wei Lan |
SpeciesList | scientific name: Arabidopsis thaliana; NCBI TaxID: 3702; |
ModificationList | ubiquitinylated lysine |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2022-04-30 06:40:33 | ID requested | |
1 | 2023-05-04 19:03:13 | announced | |
⏵ 2 | 2023-05-06 00:10:27 | announced | 2023-05-06: Update publication information. |
Publication List
Lan W, Zheng S, Yang P, Qiu Y, Xu Y, Miao Y, . Int J Mol Sci, 23(10):(2022) [pubmed] |
Lan W, Ma W, Zheng S, Yang P, Qiu Y, Lin W, Ren Y, Miao Y, -induced leaf senescence. iScience, 26(3):106216(2023) [pubmed] |
Keyword List
submitter keyword: Integrative analysis, UPL5, subcellular localization, ubiquitination, leaf senescence |
Contact List
Ying Miao | |
---|---|
contact affiliation | Fujian Provincial Key Laboratory of Functional Biology, Fujian Agriculture and Forestry University |
contact email | ymiao@fafu.edu.cn |
lab head | |
Wei Lan | |
contact affiliation | Fujian Agriculture and Forestry University |
contact email | 1150538001@fafu.edu.cn |
dataset submitter |
Full Dataset Link List
iProX dataset URI |