PXD033521-2

PXD033521 is an original dataset announced via ProteomeXchange.

Title	Multi-functions of McHB7 in Mesembryanthemum crystallinum responses to salt and drought stresses
Description	Mesembryanthemum crystallinum (common ice plant) is one of the most important halophyte plants for plant stress biology research. In this study, we established an efficient Agrobacterium-mediated transformation method in ice plant and confirmed that ice plant can sustain gene overexpression for four weeks. Expression of a salt-induced transcription factor McHB7 (OE) reached the highest level at seven days after infiltration. Under salt and drought stresses, the growth of OE was better than wild type (WT), and the activities of redox enzymes and chlorophyll contents were higher in OE than the WT. Using proteomics, 475, 510 and 378 proteins were identified to be significantly changed in the OE lines under control, salt, and drought conditions, respectively. Most increased proteins were involved in various processes including Calvin cycle, citric acid cycle, glycolysis, and antioxidant pathways. Some were found to participate in ABA biosynthesis or response. Metabolomics revealed that many metabolites and phytohormones in OE were involved in plant growth and development. Also, ABA was increased in OE lines under control, salt, and drought conditions. Yeast one-hybrid analysis showed that McHB7 can bind to ERD and ABA-related motifs. And protein-protein interaction analysis discovered the candidate proteins that were responsive to stresses and hormones (e.g., ABA).
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:40:42.402.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bowen Tan
SpeciesList	scientific name: Mesembryanthemum crystallinum (Common ice plant) (Cryophytum crystallinum); NCBI TaxID: 3544;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-28 01:23:03	ID requested
1	2024-05-21 13:01:02	announced
⏵ 2	2024-10-22 06:40:44	announced	2024-10-22: Updated project metadata.

10.3390/ijms25084569;

Zhang X, Cheng Z, Fan G, Zhu D, Tan B, Jiang T, Chen S, HB7 Improves Ice Plant Drought Tolerance through ABA Signaling Pathway. Int J Mol Sci, 25(8):(2024) [pubmed]

submitter keyword: salt stress

drought stress

proteomics

Sixue Chen
contact affiliation	Department of Biology, Genetics Institute, UF Interdisciplinary Center for Biotechnology Research (UF | ICBR),Plant Molecular and Cellular Biology Program, University of Florida
contact email	schen@ufl.edu
lab head
Bowen Tan
contact affiliation	PhD student
contact email	tanbowen@ufl.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD033521

PRIDE project URI

• PRIDE
  1. PXD033521
     1. Label: PRIDE project
     2. Name: Multi-functions of McHB7 in Mesembryanthemum crystallinum responses to salt and drought stresses