PXD033516-1

PXD033516 is an original dataset announced via ProteomeXchange.

Title	Identification of protein interaction partners of the lncRNA CALA
Description	The stimuli-responsive lncRNA CALA impacts endothelial cell function as silencing impairs angiogenic capacity in vitro. To uncover the molecular mechanism of CALA, we here combined antisense affinity selection of CALA-RNPs with mass spectrometry to identify the protein interactome of CALA. We therefore specifically enrich CALA-RNPs using two distinct antisense probes targeting CALA (LFN – probe#1, LF – probe #2) over a scrambled, non-target control (Scr/NTC) and thereby uncover CALA to interact with components of ribonucleoprotein complexes.
HostingRepository	PRIDE
AnnounceDate	2022-07-04
AnnouncementXML	Submission_2022-07-04_04:18:47.350.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ilka Wittig
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-28 01:18:38	ID requested
⏵ 1	2022-07-04 04:18:47	announced
2	2023-11-14 08:56:07	announced	2023-11-14: Updated project metadata.

10.3390/NCRNA8040049;

submitter keyword: RNA/protein interaction

Nicolas Jaé
contact affiliation	Institute for Cardiovascular Regeneration, Centre for Molecular Medicine, Goethe University Frankfurt am Main, Germany
contact email	jae@em.uni-frankfurt.de
lab head
Ilka Wittig
contact affiliation	Functional Proteomics, Goethe University, Frankfurt am Main , Germany
contact email	wittig@med.uni-frankfurt.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD033516
     1. Label: PRIDE project
     2. Name: Identification of protein interaction partners of the lncRNA CALA