PXD033514 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Dexamethasone is a regulator of human testicular peritubular cells / Proteome 24 h |
Description | The functions of human testicular peritubular cells (HTPCs), which form a small compartment located between the seminiferous epithelium and the interstitials areas of the testis, go beyond intratesticular sperm transport and include immunological roles. The expression of the glucocorticoid receptor (GR/NR3C1) by these cells indicates that they are a target of glucocorticoids (GCs), yet detailed insights into consequences of GC actions are unknown. To address this point, we studied cultured HTPCs, which serve as a window into the human testis. We used a cytokine profiler assay to screen for changes in secreted cytokines upon dexamethasone (Dex) treatment and employed qPCR and ELISA studies to verify the results. Dex, used in a therapeutic concentration, decreased proinflammatory factors, including IL6, IL8, MCP1 and CXCL1 within 24 - 48 h. An siRNA-mediated knockdown of GR reduced these actions. Exposure to Dex resulted in reduced GR levels, implying that exposure to Dex could also reduce anti-inflammatory actions of Dex. To evaluate the influence of Dex on HTPCs further, we performed a proteomic analysis of cellular components and secreted proteins. Strong responses were detectable after 24 h (31 significantly altered cellular proteins) and more pronounced ones after 72 h (30 less abundant and 42 more abundant cellular proteins). Dex also altered the composition of the secretome (33 proteins decreased, 13 increased) after 72 h. Among the regulated proteins were ECM- and basement membrane components (e.g. FBLN2, COL1A2, COL3A1), as well as PTX3 and StAR. These results reveal that Dex via GR exerts profound actions in HTPCs. If transferrable to the human testis, changes specifically in ECM and the immunological state of the testis may result in men upon treatment with Dex for medical reasons. -Proteome Data Subset 24 h- |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:43:13.492.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Youli Konstantinovitch Stepanov |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive HF-X |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-04-28 01:18:23 | ID requested | |
1 | 2023-03-11 06:51:15 | announced | |
⏵ 2 | 2023-11-14 08:43:17 | announced | 2023-11-14: Updated project metadata. |
Publication List
Stepanov YK, Speidel JD, Herrmann C, Schmid N, Behr R, K, ö, hn FM, St, ö, ckl JB, Pickl U, Trottmann M, Fr, ö, hlich T, Mayerhofer A, Welter H, Profound Effects of Dexamethasone on the Immunological State, Synthesis and Secretion Capacity of Human Testicular Peritubular Cells. Cells, 11(19):(2022) [pubmed] |
Keyword List
submitter keyword: Human male fertility |
human testis |
dexamethasone |
cytokines |
inflammation |
proteomics |
Contact List
Dr. Thomas Fröhlich |
contact affiliation | Laboratory for Functional Genome Analysis LAFUGA, Gene Center, LMU München, München, Germany, Feodor-Lynen-Straße 25, 81377 München |
contact email | frohlich@genzentrum.lmu.de |
lab head | |
Youli Konstantinovitch Stepanov |
contact affiliation | LMU München Genzentrum-LAFUGA |
contact email | Stepanov@genzentrum.lmu.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD033514
- Label: PRIDE project
- Name: Dexamethasone is a regulator of human testicular peritubular cells / Proteome 24 h