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PXD033470 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTryptophan C-mannosylation is critical for Plasmodium falciparum transmission Dataset1
DescriptionC-mannosylation stabilizes proteins bearing a thrombospondin repeat (TSR) domain in metazoans. Here we show that Plasmodium falciparum expresses a DPY19 C-mannosyltransferase in the endoplasmic reticulum and that DPY19-deficiency abolishes C-glycosylation, destabilizes members of the TRAP adhesin family and inhibits transmission to mosquitoes. P. falciparum gametogenesis was imaged in its entirety in four dimensions using lattice light-sheet microscopy. This revealed defects in egress and exflagellation for DPY19 microgametes. While exflagellation was diminished, DPY19 microgametes still fertilized macrogametes, forming ookinetes but these were abrogated for mosquito infection. The gametogenesis defects corresponded with destabilization of MTRAP, which we show is C-mannosylated in P. falciparum, and the ookinete defect was concordant with defective CTRP secretion on the DPY19 background. Genetic complementation of DPY19 restored ookinete infectivity, sporozoite production and C-mannosylation activity. Therefore, tryptophan C-mannosylation by DPY19 in the early secretory pathway ensures TSR protein quality control at two lifecycle stages for successful transmission of the human malaria parasite.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterNichollas Scott
SpeciesList scientific name: Plasmodium falciparum; NCBI TaxID: 5833;
ModificationListcomplex glycosylation
InstrumentOrbitrap Eclipse
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-04-27 03:47:26ID requested
12022-07-06 14:49:38announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Plasmodium falciparum, transmission, glycosylation, gametocyte, gamete, ookinete, sporozoite, DPY19
Contact List
Justin A. Boddey
contact affiliation-The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Victoria 3052, Australia. -Department of Medical Biology, University of Melbourne, Parkville, Victoria 3010, Australia.
contact emailboddey@wehi.edu.au
lab head
Nichollas Scott
contact affiliationUniversity of Melbourne
contact emailnichollas.scott@unimelb.edu.au
dataset submitter
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Dataset FTP location
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