PXD033451-2

PXD033451 is an original dataset announced via ProteomeXchange.

Title	Human P47 interactome for comparison against CryoEM data
Description	p97 is an essential and abundant AAA+ ATPase that unfolds ubiquitylated substrates and is a central regulator of protein homeostasis. Here we report two cryo-EM structures of human p97 in complex with its p47 adaptor. One of the conformations is six-fold symmetric, corresponds to previously reported structures of p97, and lacks bound substrate. The other structure adopts a helical conformation, displays substrate running in an extended conformation through the pore of the p97 hexamer, and resembles structures reported for other AAA unfoldases. These findings support the model that p97 utilizes a “hand-over-hand” mechanism in which two residues of the substrate are translocated for hydrolysis of two ATPs, one in each of the two p97 AAA ATPase rings. Proteomics analysis supports the model that one p97 complex can bind multiple substrate adaptors or binding partners, and can process substrates with multiple types of ubiquitin modification
HostingRepository	PRIDE
AnnounceDate	2022-05-16
AnnouncementXML	Submission_2022-05-16_05:58:10.150.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD033451
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	John Price
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-25 04:27:18	ID requested
1	2022-05-16 05:50:58	announced
⏵ 2	2022-05-16 05:58:10	announced	2022-05-16: Updated publication reference for PubMed record(s): 35552390.

Xu Y, Han H, Cooney I, Guo Y, Moran NG, Zuniga NR, Price JC, Hill CP, Shen PS, Active conformation of the p97-p47 unfoldase complex. Nat Commun, 13(1):2640(2022) [pubmed]

submitter keyword: Flag, affinity purification, HEK293

John C. Price
contact affiliation	Brigham Young University
contact email	drjohncprice@gmail.com
lab head
John Price
contact affiliation	Brigham Young University
contact email	drjohncprice@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD033451

PRIDE project URI

• PRIDE
  1. PXD033451
     1. Label: PRIDE project
     2. Name: Human P47 interactome for comparison against CryoEM data