PXD033442-2

PXD033442 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Comparative Proteomic Analysis of Seminal Plasma Exosomes in Buffalo with High and Low Sperm Motility
Description	Exosomes are nanosized membranous vesicles secreted by variety types of cells and regulate intercellular communication through deliver bioactive substances. They are found in abundance in biological fluids including semen. Although protein composition and potential fertility biomarkers of seminal plasma exosomes (SPE) have been identified in human, but they remains unclear in buffalo. Here, we aim to discover the protein correlation between spermatozoa, seminal plasma (SP) and SPE, then further compare and analyze the protein differences between high and low motility SPEs in buffalo. SPEs were concentrated and purified by ultracentrifugation combined by sucrose density gradient centrifugation, followed by verification by western blot, nanoparticle tracking analysis, and transmission electron microscopy. Protein composition in spermatozoa, SP and SPE, and protein difference in high and low motility SPEs were identified by LC-MS/MS proteomic analysis and functional analyzed by a comprehensive bioinformatic analysis.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:23:44.028.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	qiang Fu
SpeciesList	scientific name: Bubalus bubalis; NCBI TaxID: 89462;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-25 04:05:17	ID requested
1	2022-12-05 04:11:36	announced
⏵ 2	2023-11-14 08:23:46	announced	2023-11-14: Updated project metadata.

Publication List

Fu Q, Pan L, Huang D, Wang Z, Hou Z, Zhang M, Proteomic profiles of buffalo spermatozoa and seminal plasma. Theriogenology, 134():74-82(2019) [pubmed]

Fu Q, Pan L, Huang D, Wang Z, Hou Z, Zhang M, Proteomic profiles of buffalo spermatozoa and seminal plasma. Theriogenology, 134():74-82(2019) [pubmed]

Keyword List

ProteomeXchange project tag: Human Proteome Project
submitter keyword: buffalo
seminal plasma
exosomes
proteome
activity
sperm motility

ProteomeXchange project tag: Human Proteome Project

submitter keyword: buffalo

seminal plasma

exosomes

proteome

activity

sperm motility

Contact List

Qiang Fu
contact affiliation	State Key Laboratory for Conservation and Ulilization of Subtropical Agro-bioresource
contact email	gxfuq@163.com
lab head
qiang Fu
contact affiliation	Guangxi university, state key lab for subtropical Agro-bioresource conservation and utilization, SKLCUSA
contact email	gxfuq@163.com
dataset submitter

Full Dataset Link List

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PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/12/PXD033442

PRIDE project URI

Repository Record List

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