PXD033441 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Development of an in vitro screening system for synthetic signal peptide in mammalian cell-based protein production. |
Description | Optimizing appropriate signal peptides in mammalian cell-based protein production is crucial given that most recombinant proteins produced in mammalian cells are thought to be secreted proteins. Until now, most studies on signal peptide in mammalian cells have replaced native signal peptides with well-known heterologous signal peptides and bioinformatics-based signal peptides. In the present study, we successfully established an in vitro screening system for synthetic signal peptide in CHO cells by combining a degenerate codon-based oligonucleotides library, a site-specific integration system, and a FACS-based antibody detection assay. Three new signal peptides were screened using this new screening system, confirming to have structural properties as signal peptides by the SignalP web server, a neural network-based algorithm that quantifies the signal peptide-ness of amino acid sequences. The novel signal peptides selected in this study increased Fc-fusion protein production in CHO cells by increasing specific protein productivity, while it did not negatively affect cell growth. In addition, replacing native signal peptide with the novel signal peptides did not significantly affect sialylated N-glycan formation, N-terminal cleavage pattern, and biological function of Fc-fusion protein produced in CHO cells. The overall results indicate the utility of a novel in vitro screening system for synthetic signal peptide for mammalian cell-based protein production. |
HostingRepository | PRIDE |
AnnounceDate | 2022-05-24 |
AnnouncementXML | Submission_2022-05-23_23:56:52.150.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jong-Ho Park |
SpeciesList | scientific name: Cricetulus griseus; NCBI TaxID: 10029; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive Plus |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-04-25 01:54:19 | ID requested | |
⏵ 1 | 2022-05-23 23:56:52 | announced | |
2 | 2023-11-14 08:56:07 | announced | 2023-11-14: Updated project metadata. |
Publication List
Keyword List
ProteomeXchange project tag: Biology/Disease-Driven Human Proteome Project (B/D-HPP) |
submitter keyword: Signal peptide, Mammalian cells, Screening system, Site-specific integration system, Fc-fusion protein production, N-terminal sequencing using LC-MS/MS |
Contact List
Yeon-Gu Kim |
contact affiliation | Biotherapeutics Translational Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon, Korea |
contact email | ygkim@kribb.re.kr |
lab head | |
Jong-Ho Park |
contact affiliation | Korea Research Institute of Bioscience and Biotechnology (KRIBB) |
contact email | parkjong123@kribb.re.kr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD033441
- Label: PRIDE project
- Name: Development of an in vitro screening system for synthetic signal peptide in mammalian cell-based protein production.