PXD033429 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Global bottom-up analysis of wild type and RBM20 knock out rat hearts by LC-TIMS-MSMS |
Description | Dilated cardiomyopathy (DCM) is a major risk factor for developing heart failure and is often associated with an increased risk for life-threatening arrhythmia. Although numerous causal genes for DCM have been identified, RNA binding motif 20 (RBM20) remains one of the few splicing factors that, when mutated or genetically ablated, leads to the development of DCM. In this study we sought to identify changes in the cardiac proteome in RBM20 deficient rat hearts using global quantitative proteomics to gain insight into the molecular mechanisms precipitating the development of DCM secondary to RBM20 loss. Our analysis identified changes in titin interacting proteins, as well as mitochondrial enzymes, implicating activation of pathological hypertrophy and mitochondrial dysfunction in DCM development in RBM20 deficient rats. Collectively, our findings provide the first look into changes in the cardiac proteome associated with genetic ablation of RBM20. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:56:02.212.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Eli Larson |
SpeciesList | scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116; |
ModificationList | phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | timsTOF Pro |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-04-24 12:50:06 | ID requested | |
1 | 2023-05-12 11:16:13 | announced | |
⏵ 2 | 2023-11-14 08:56:02 | announced | 2023-11-14: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Rat, RBM20, Heart, timsTOF Pro |
Contact List
Ying Ge |
contact affiliation | Department of Cell and Regenerative Biology, Department of Chemistry, University of Wisconsin-Madison |
contact email | ying.ge@wisc.edu |
lab head | |
Eli Larson |
contact affiliation | University of Wisconsin-Madison |
contact email | ejlarson2@wisc.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD033429 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD033429
- Label: PRIDE project
- Name: Global bottom-up analysis of wild type and RBM20 knock out rat hearts by LC-TIMS-MSMS