PXD033394-1

PXD033394 is an original dataset announced via ProteomeXchange.

Title	Angel2 phosphatase activity is required for non-canonical mitochondrial RNA processing
Description	Canonical RNA processing in mammalian mitochondria is defined by tRNAs acting as recognition sites for nucleases to release flanking transcripts. The relevant factors, their structures, and mechanism are well described, but not all mitochondrial transcripts are punctuated by tRNAs, and their mode of processing has remained unsolved. Using Drosophila and mouse models, we demonstrate that non-canonical processing results in the formation of 3′ phosphates, and that phosphatase activity by the carbon catabolite repressor 4 domain-containing family member ANGEL2 is required for their hydrolysis. Furthermore, our data suggest that members of the FAST kinase domain-containing protein family are responsible for these 3′ phosphates. Our results therefore resolve the mechanism of non-canonical RNA processing in metazoan mitochondria, by defining the role of ANGEL2.
HostingRepository	PRIDE
AnnounceDate	2022-09-01
AnnouncementXML	Submission_2022-09-01_07:46:37.596.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Florian Schober
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-23 15:33:22	ID requested
⏵ 1	2022-09-01 07:46:37	announced

Dataset with its publication pending

submitter keyword: Mitochondria, RNA, processing, Angel2, Drosophila, Mouse

Anna Wredenberg
contact affiliation	Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Solna/Sweden
contact email	anna.wredenberg@ki.se
lab head
Florian Schober
contact affiliation	MMK, Karolinska Institute
contact email	florian.schober@ki.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/09/PXD033394

PRIDE project URI

• PRIDE
  1. PXD033394
     1. Label: PRIDE project
     2. Name: Angel2 phosphatase activity is required for non-canonical mitochondrial RNA processing