PXD033387-1

PXD033387 is an original dataset announced via ProteomeXchange.

Title	Interactions between paralogous bacterial enhancer binding proteins enable metal-dependent regulation of alternative nitrogenases in Azotobacter vinelandii
Description	All diazotrophic bacteria and archaea isolated so far utilise a nitrogenase enzyme containing molybdenum in the active site co-factor to fix atmospheric dinitrogen to ammonia. However, in addition to the Mo-dependent nitrogenase, some nitrogen-fixing prokaryotes also express genetically distinct alternative nitrogenase isoenzymes, namely the V-dependent and Fe-only nitrogenases respectively. Nitrogenase isoenzymes are expressed hierarchically according to metal availability and catalytic efficiency. In proteobacteria, this hierarchy is maintained via stringent transcriptional regulation of gene clusters by dedicated bacterial enhancer binding proteins (bEBPs). The model diazotroph Azotobacter vinelandii contains two paralogs of the vanadium nitrogenase activator VnfA (henceforth, VnfA1), designated VnfA2 and VnfA3, with unknown functions. Here we demonstrate that the VnfA1 and VnfA3 bEBPs bind to the same target promoters in the Azotobacter vinelandii genome and co-activate a subset of genes in the absence of V, including the structural genes for the Fe only nitrogenase. Co-activation is inhibited by the presence of V and is dependent on an accessory protein VnfZ that is co-expressed with VnfA3. Our studies uncover a plethora of interactions between bEBPs required for nitrogen fixation, revealing unprecedented potential for fine tuning expression of alternative nitrogenases in response to metal availability.
HostingRepository	PRIDE
AnnounceDate	2022-06-23
AnnouncementXML	Submission_2022-06-23_07:48:31.444.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD033387
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Carlo de Oliveira Martins
SpeciesList	scientific name: Azotobacter vinelandii DJ; NCBI TaxID: 322710;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-23 15:27:33	ID requested
⏵ 1	2022-06-23 07:48:31	announced

Dataset with its publication pending

submitter keyword: Azotobacter, alternative nitrogenases, Co-IP, LC-MSMS

Ray Dixon
contact affiliation	Group Leader, Department of Molecular Microbiology
contact email	Ray.Dixon@jic.ac.uk
lab head
Carlo de Oliveira Martins
contact affiliation	John Innes Centre
contact email	Carlo.Martins@jic.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD033387
     1. Label: PRIDE project
     2. Name: Interactions between paralogous bacterial enhancer binding proteins enable metal-dependent regulation of alternative nitrogenases in Azotobacter vinelandii