PXD033369-1

PXD033369 is an original dataset announced via ProteomeXchange.

Title	An integral atlas of hormone peptides of human EECs and their dedicated proteases
Description	Enteroendocrine cells (EECs) secrete hormones in response to ingested nutrients to control physiological processes such as appetite and insulin release. EEC hormones are synthesized as large pre-proteins that undergo proteolytic processing through peptidases to generate bioactive peptides. Due to the rarity of EECs and lack of cell models, prohormone processing remains challenging to assess. Here, we generated a panel of human organoid models in which EECs can efficiently be induced, combined with CRISPR-Cas9-mediated genetic inactivation or alteration of peptidases. We employed mass spectrometry based analysis to monitor peptide processing in knock-outs and wild type organoids for various hormone-processing enzymes (including PCSK1, PCSK2, PCSK1N, PCSK1mut_S375G, PCSK2mut_R430W, DPP4, CPB1 and CPE). We identify PCSK2-dependent Glucagon production in EECs, which is stimulated by activation of BMP signaling. We further map the substrates of other major EECs endo- and exopeptidases.
HostingRepository	PRIDE
AnnounceDate	2022-11-26
AnnouncementXML	Submission_2022-11-26_04:35:37.795.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	JuliaBauzá Martinez
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	amidated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-22 05:45:42	ID requested
⏵ 1	2022-11-26 04:35:38	announced
2	2023-11-14 08:56:03	announced	2023-11-14: Updated project metadata.

submitter keyword: peptidomics, LC-MSMS, proteolytic processing,hormones, enteroendocrine cells

WeiWu
contact affiliation	Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands Singapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR), Singapore, Singapore. Department of Pharmacy, National University of Singapore, Singapore 117543, Singapore.
contact email	wu_wei@immunol.a-star.edu.sg
lab head
JuliaBauzá Martinez
contact affiliation	Utrecht University
contact email	j.bauzamartinez@uu.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/11/PXD033369

PRIDE project URI

• PRIDE
  1. PXD033369
     1. Label: PRIDE project
     2. Name: An integral atlas of hormone peptides of human EECs and their dedicated proteases