PXD033361 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Investigating the Consistency of Extracellular Vesicle Production from Breast Cancer Subtypes Using CELLine Adherent Bioreactors |
| Description | Extracellular vesicle (EV) research has grown rapidly in recent years, largely due to the potential use of EVs as liquid biopsy biomarkers or therapeutics. However, in-depth characterisation and validation of EVs produced using conventional in vitro cultures can be challenging due to the large area of cell monolayers and volumes of culture media required. To overcome this obstacle, multiple bioreactor designs have been tested for EV production with varying success, but the consistency of EVs produced over time has not been reported previously. In this study, we demonstrate that several breast cancer cell lines of different subtypes can be cultured simultaneously in space, resource, and time efficient manner using CELLine AD 1000 systems, allowing the consistent production of vast amounts of EVs for downstream experimentation. We report an improved workflow used for inoculating, maintaining, and monitoring the bioreactors, their EV production, and the characterisation of the EVs produced. Lastly, our proteomic analyses of the EVs produced throughout the lifetime of the bioreactors show that core EV-associated proteins are relatively consistent, with few minor variations over time, and that tracking the production of EVs may be a convenient method for indirectly monitoring the bioreactors’ health. These findings will aid future studies requiring the simultaneous production of large amounts of EVs from several cell lines of different subtypes of a disease and other EV biomanufacturing applications. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-08-04 |
| AnnouncementXML | Submission_2025-08-03_16:15:32.837.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Bincy Jacob |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
| ModificationList | iodoacetamide derivatized residue |
| Instrument | TripleTOF 6600 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2022-04-21 07:17:36 | ID requested | |
| ⏵ 1 | 2025-08-03 16:15:33 | announced | |
Publication List
Keyword List
| submitter keyword: CELLine bioreactor, breast cancer, exosomes, proteomics, microvesicles,Extracellular vesicles |
Contact List
| Cherie Blenkiron |
|---|
| contact affiliation | FMHS Building 501 Level 2, Room 210 85 Park Rd Grafton Auckland 1023 New Zealand |
| contact email | c.blenkiron@auckland.ac.nz |
| lab head | |
| Bincy Jacob |
|---|
| contact affiliation | The University of Auckland |
| contact email | bjac907@aucklanduni.ac.nz |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD033361
- Label: PRIDE project
- Name: Investigating the Consistency of Extracellular Vesicle Production from Breast Cancer Subtypes Using CELLine Adherent Bioreactors
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