PXD033234-1

PXD033234 is an original dataset announced via ProteomeXchange.

Title	CryoEM structure of mitochondrial complex I from the thermophilic eukaryote Chaetomium thermophilum
Description	Mitochondrial complex I is a redox-driven proton pump that generates most of the proton-motive force powering oxidative phosphorylation and ATP synthesis in eukaryotes. We report the structure of complex I from the thermophilic eukaryote Chaetomium thermophilum, determined by electron cryo-microscopy to 2.4 A resolution in the open and closed conformation. Complex I has two arms, the peripheral and membrane arm, forming an L-shape. The two conformations differ in the relative position of the two arms. The open-to-closed transition is accompanied by substantial conformational changes in the Q-binding cavity and the E-channel, and by the formation of an aqueous connection between the E-channel and an extensive aqueous passage inside the membrane arm. The observed similarities provide strong support for a conserved, common mechanism that applies across all species from fungi to mammals. Furthermore, the complex is inhibited by the detergent DDM, which binds reversibly to two sites in the Q-binding cavity.
HostingRepository	PRIDE
AnnounceDate	2022-11-29
AnnouncementXML	Submission_2022-11-29_01:34:30.156.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	JulianLanger
SpeciesList	scientific name: Chaetomium thermophilum; NCBI TaxID: 209285;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-14 05:07:32	ID requested
⏵ 1	2022-11-29 01:34:30	announced
2	2023-11-14 08:54:13	announced	2023-11-14: Updated project metadata.

submitter keyword: mitochondrial complex I, Chaetomium thermophilum

JulianLanger
contact affiliation	Max Planck Institute of Biophysics, Max Planck Institute for Brain Research
contact email	julian.langer@biophys.mpg.de
lab head
JulianLanger
contact affiliation	MPIs for Biophysics and Brain Research
contact email	julian.langer@biophys.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD033234
     1. Label: PRIDE project
     2. Name: CryoEM structure of mitochondrial complex I from the thermophilic eukaryote Chaetomium thermophilum