PXD033231-1

PXD033231 is an original dataset announced via ProteomeXchange.

Title	Aedes aegypti Aag-2 cells proteome modulation in response to Chikungunya virus infection
Description	Chikungunya virus (CHIKV) is a single-stranded positive RNA virus that belongs to the genus Alphavirus and is transmitted to humans by infected Aedes aegypti and Aedes albopictus bites. In humans, CHIKV can cause painful symptoms during acute and chronic stages of infection. However, the virus-vector interaction has characteristics that allow a persistent infection, not disturbing the mosquito’s fitness. Here, we aimed to clarify aspects of CHIKV infection in Ae. aegypti Aag-2 cells through label-free quantitative proteomic analysis and transmission electron microscopy (TEM). We used MOI 0.1 to infect Aag-2 cells in biological triplicates over 48 h. TEM images show a high load of intracellular viral cargo at 48 hpi, as well as an elongated unusual mitochondria morphology that might indicate a mitochondrial imbalance. Moreover, a total of 196 Ae. aegypti protein groups were up or downregulated upon infection, related to protein synthesis, energy metabolism, signaling pathways and apoptosis. These regulated Aag-2 proteins might have roles in antiviral and/or in pro-viral mechanisms during CHIKV infection, to support the balance between viral propagation and the survival of host cell, leading to the persistent infection.
HostingRepository	PRIDE
AnnounceDate	2022-06-02
AnnouncementXML	Submission_2022-06-02_06:26:22.725.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	SEBASTIEN CHARNEAU
SpeciesList	scientific name: Aedes aegypti; NCBI TaxID: 7159; scientific name: Chikungunya virus strain S27-African prototype; NCBI TaxID: 371094;
ModificationList	L-selenocysteine (Cys); monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-14 04:45:33	ID requested
⏵ 1	2022-06-02 06:26:23	announced

Dataset with its publication pending

submitter keyword: Chikungunya virus, mosquito, label-free quantification, mass spectrometry, mitochondria, protein synthesis, apoptosis, RNA helicases.

Sébastien Charneau
contact affiliation	Laboratory of Protein Chemistry and Biochemistry, Institute of Biology, Department of Cell Biology, University of Brasília, Brasília, Brazil
contact email	charneau@unb.br
lab head
SEBASTIEN CHARNEAU
contact affiliation	UNIVERSITY OF BRASILIA
contact email	charneau@unb.br
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD033231
     1. Label: PRIDE project
     2. Name: Aedes aegypti Aag-2 cells proteome modulation in response to Chikungunya virus infection