PXD033226-1

PXD033226 is an original dataset announced via ProteomeXchange.

Title	Enrichment and Fragmentation Approaches for Enhanced Detection and Characterization of Endogenous Glycosylated Neuropeptides
Description	Glycosylation, a common and heterogeneous post-translational modification plays a key role in altering biomolecule function and other properties. Prior to monitoring regulatory roles, basal glyconeuropeptide expression must be determined. Characterization is partially limited by the lower abundance of glycosylated neuropeptides in vivo compared to non-modified neuropeptides and thus requires effective enrichment and characterization strategies. A hydrophilic interaction liquid chromatography enrichment strategy is modified, and fragmentation schemes are evaluated to establish a workflow for future glyconeuropeptide studies to improve the understanding of neuropeptide glycosylation, as well as its distribution across the neuroendocrine system and several neuropeptide families. Product ion-triggered electron-transfer/higher-energy collision dissociation and stepped collision energy higher-energy collisional dissociation are evaluated.
HostingRepository	PRIDE
AnnounceDate	2022-08-19
AnnouncementXML	Submission_2022-08-19_13:19:53.238.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ashley Phetsanthad
SpeciesList	scientific name: Callinectes sapidus; NCBI TaxID: 6763;
ModificationList	amidated residue; glycosylated residue
Instrument	Orbitrap Fusion Lumos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-14 04:29:08	ID requested
⏵ 1	2022-08-19 13:19:53	announced
2	2023-11-14 08:54:13	announced	2023-11-14: Updated project metadata.

10.1002/PMIC.202100375;

submitter keyword: de novo sequencing, endogenous peptides, glycosylation, mass spectrometry, neuropeptides

Lingjun Li
contact affiliation	School of Pharmacy and Department of Chemistry, Lingjun Li Lab, University of Wisconsin - Madison, USA
contact email	lingjun.li@wisc.edu
lab head
Ashley Phetsanthad
contact affiliation	University of Wisconsin - Madison
contact email	ashphets@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD033226
     1. Label: PRIDE project
     2. Name: Enrichment and Fragmentation Approaches for Enhanced Detection and Characterization of Endogenous Glycosylated Neuropeptides