PXD033120-1

PXD033120 is an original dataset announced via ProteomeXchange.

Title	The protein phosphatase 2A holoenzyme is a key regulator of starch metabolism and bradyzoite differentiation in Toxoplasma gondii
Description	Phenotypic switching from tachyzoite to bradyzoite and vice versa is the fundamental mechanism underpinning the pathogenicity and adaptability of the protozoan parasite Toxoplasma gondii. Accumulation of cytoplasmic starch granules is a hallmark of the quiescent bradyzoite stage. The regulatory factors and mechanisms that contribute to amylopectin storage in bradyzoites remain incompletely known. Here, we show that T. gondii protein phosphatase 2A (PP2A) holoenzyme is composed of a catalytic subunit (PP2A-C), a structural subunit (PP2A-A) and a regulatory subunit (PP2A-B). Disruption of any of these subunits increased starch accumulation and disrupted the parasite differentiation. The putative PP2A holoenzyme substrates were identified by phosphoproteomics. PP2A contributes to the regulation of amylopectin metabolism via dephosphorylation of calcium-dependent protein kinase 2 at S679. Several putative PP2A substrates were found to play important roles in bradyzoite differentiation. Our findings establish PP2A as an integral component of the regulatory network mediating amylopectin metabolism and tachyzoite-bradyzoite transformation in T. gondii.
HostingRepository	PRIDE
AnnounceDate	2022-11-05
AnnouncementXML	Submission_2022-11-05_05:55:43.505.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	JinLeiWang
SpeciesList	scientific name: Toxoplasma gondii; NCBI TaxID: 5811;
ModificationList	phosphorylated residue
Instrument	LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-08 13:38:32	ID requested
⏵ 1	2022-11-05 05:55:43	announced

Dataset with its publication pending

submitter keyword: PP2A holoenzyme,Toxoplasma gondii, differentiation, dephosphorylation., starch metabolism

Xing-QuanZhu
contact affiliation	State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province 730046, PR China
contact email	xingquanzhu1@hotmail.com
lab head
JinLeiWang
contact affiliation	Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
contact email	wangjinlei90@126.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD033120
     1. Label: PRIDE project
     2. Name: The protein phosphatase 2A holoenzyme is a key regulator of starch metabolism and bradyzoite differentiation in Toxoplasma gondii