PXD033118-1

PXD033118 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	K48-Ubiquitin IP-MS of Jurkat leukemia cells treated with asparaginase
Description	We have shown that drug-resistant leukemias rely on protein degradation through the ubiquitin proteasome pathway to survive during asparaginase therapy. Our studies support the model that this catabolic source of amino acids is upregulated in response to amino acid starvation. To investigate whether this involves bulk protein degradation or the degradation of specific protein targets, we analyzed K48-ubiquitinated proteins using immunoprecipitation with an anti-K48-linked ubiquitin antibody (Millipore #05-1307) followed by mass spectrometry proteomics from Jurkat cells after treatment with vehicle vs asparaginase. We found no enrichment of specific proteins after asparaginase treatment, consistent with the possibility that asparaginase therapy stimulates bulk protein degradation, although these results do not rule out degradation of specific proteins below the limit of detection of this assay.
HostingRepository	MassIVE
AnnounceDate	2022-04-12
AnnouncementXML	Submission_2022-04-12_14:59:28.635.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Alejandro Gutierrez
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-08 11:57:51	ID requested
⏵ 1	2022-04-12 14:59:28	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: Asparaginase, leukemia, ubiquitin

submitter keyword: Asparaginase, leukemia, ubiquitin

Contact List

Alejandro Gutierrez
contact affiliation	Boston Children's Hospital
contact email	alejandro.gutierrez@childrens.harvard.edu
lab head
Alejandro Gutierrez
contact affiliation	Boston Children's Hospital
contact email	alejandro.gutierrez@childrens.harvard.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000089226/