PXD033108-1

PXD033108 is an original dataset announced via ProteomeXchange.

Title	Discovery and validation of serum glycoprotein biomarker panel for detection of high grade serous ovarian cancer
Description	We aimed to discover and validate a panel of serum biomarkers for high grade serous ovarian cancer (HGSOC) using our lectin-magnetic bead array-coupled proteomics platform. Serum from age-matched women with HGSOC, benign tumours or healthy controls were analysed in discovery (UKCTOCS, n=30 and UKOPS, n=30) and validation (Australian Ovarian Cancer Study, n=95) cohorts using shotgun and targeted proteomics, respectively. A 7-lectin discovery screen shortlisted 60 candidate proteins and 3 lectins for validation, which revealed elevated levels of AAL, SNA or STL-binding FIBB, CO9, ITIH3, HPT, A1AT, AACT in HGSOC, while IBP3, A2MG, PON1, CHLE and ALS were reduced. Multimarker panels were developed using generalized regression with lasso estimation and leave-one-out cross-validation. The best performing panel comprised of 13 peptides from Solanum Tuberosum lectin (STL)-binding proteins with 96.3% area under the receiver operating curve, 97.7% specificity and 78.6% sensitivity for distinguishing HGSOC from benign and healthy groups. The peptides robust in cross-validations were from IBP3, KNG1, CO9, THRB, HPTR, HPT, FINC, FA10, GELS. The validated serum biomarkers show promise for early detection of HGSOC and should be further evaluated.
HostingRepository	PanoramaPublic
AnnounceDate	2024-08-06
AnnouncementXML	Submission_2024-08-06_14:23:54.105.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	supported by repository but incomplete data and/or metadata
PrimarySubmitter	Mriga Dutt
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	Carbamidomethyl; Label:13C(6)15N(2); Label:13C(6)15N(4); Label:13C(5)15N(1)
Instrument	6490 Triple Quadrupole LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-08 09:31:12	ID requested
⏵ 1	2024-08-06 14:23:54	announced

Dutt M, Hartel G, Richards RS, Shah AK, Mohamed A, Apostolidou S, Gentry-Maharaj A, Hooper JD, Perrin LC, Menon U, Hill MM, Discovery and validation of serum glycoprotein biomarkers for high grade serous ovarian cancer. Proteomics Clin Appl, 17(4):e2200114(2023) [pubmed]

submitter keyword: glycosylation, LeMBA, lectin, ovarian cancer screening, STL

Michelle Hill
contact affiliation	QIMR Berghofer Medical Research Institute
contact email	michelle.hill@qimrberghofer.edu.au
lab head
Mriga Dutt
contact affiliation	QIMR Berghofer Medical Research Institute
contact email	dutt.mriga@gmail.com
dataset submitter

Panorama Public dataset URI