PXD033080-3

PXD033080 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Identification of novel prognostic indicators for oral squamous cell carcinoma based on proteomics
Description	Lysate (1.5% SDS / 100 mm Tris-Cl) was added to samples on ice, mixed well and centrifuged to obtain supernatant. The protein concentration was determined by Bradford assay, and the protein bands were visualized by SDS-PAGE. Enzymatic digestion with trypsin was followed by Sep-Pak C18 desalting. Equal amounts of samples were taken for TMT labeling, which was performed according to the manufacturer's instructions. After equal mixing of the labeled samples, the pooled samples were fractionated by high pH reverse chromatography and checked on the machine. Mass spectrometry data were acquired using a liquid-mass coupled system with an Orbitrap Exploris 480 mass spectrometer in tandem with an EASY-nLC 1200 liquid phase. Mass spectrometric data were searched by MaxQuant (v1.6.6) software and the adopted database searching algorithm was Andromeda. The database used for the search was Swissprot Human (20210312) proteome reference databases.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:55:00.371.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhitao Yao
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	LTQ Orbitrap

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-07 03:55:02	ID requested
1	2023-07-20 10:52:22	announced
2	2023-11-14 09:02:55	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 05:55:01	announced	2024-10-22: Updated project metadata.

Publication List

10.1016/j.tranon.2023.101672;
Yao Z, An W, Tuerdi M, Zhao J, Identification of novel prognostic indicators for oral squamous cell carcinoma based on proteomics and metabolomics. Transl Oncol, 33():101672(2023) [pubmed]

10.1016/j.tranon.2023.101672;

Yao Z, An W, Tuerdi M, Zhao J, Identification of novel prognostic indicators for oral squamous cell carcinoma based on proteomics and metabolomics. Transl Oncol, 33():101672(2023) [pubmed]

Keyword List

submitter keyword: proteomics,oral squamous cell carcinoma

submitter keyword: proteomics,oral squamous cell carcinoma

Contact List

Zhitao Yao
contact affiliation	Department of Trauma and Orthopedics, the First Affiliated Hospital of Xinjiang Medical University
contact email	yaozhitaodoctor@163.com
lab head
Zhitao Yao
contact affiliation	Department of Trauma and Orthopedics, the First Affiliated Hospital of Xinjiang Medical University
contact email	2831471276@qq.com
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/07/PXD033080
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/07/PXD033080

PRIDE project URI

Repository Record List

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