PXD033046-2

PXD033046 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Pseudolycoriella hygida saliva proteome
Description	This experiment aimed at indentifying secreted proteins/enzymes from saliva of the fly Pseudolycoriella hygida. To do this, twelve-day-old Psl. hygida larvae were selected and the last segment of the larvae was tied with elastane fishing line with the help of a stereomicroscope. This was performed to avoid contamination of saliva samples with feaces. Groups of 20 tied larvae were transferred to 1.5-mL microtubes and a few holes were made in the microtube cap. Then, the microtubes were incubated at 22 °C for 3 h. After removing the larvae, the microtubes were briefly centrifuged at 15,000 g for 30 s, and the secreted saliva, usually 2-3 ul per tube, was stored at -20 °C until gel electrophoresis.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:44:40.237.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Richard Ward
SpeciesList	scientific name: Bradysia hygida; NCBI TaxID: 35572;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-07 02:55:31	ID requested
1	2023-04-07 13:10:49	announced
⏵ 2	2023-11-14 08:44:43	announced	2023-11-14: Updated project metadata.

Publication List

Trinca V, Carli S, Uliana JVC, Garbelotti CV, Mendes da Silva M, Kunes V, Meleiro LP, Brancini GTP, Menzel F, Andrioli LPM, Torres TT, Ward RJ, Monesi N, CAZymes (Sciaroidea, Diptera) in degrading plant and fungal cell wall polysaccharides. iScience, 26(4):106449(2023) [pubmed]

Trinca V, Carli S, Uliana JVC, Garbelotti CV, Mendes da Silva M, Kunes V, Meleiro LP, Brancini GTP, Menzel F, Andrioli LPM, Torres TT, Ward RJ, Monesi N, CAZymes (Sciaroidea, Diptera) in degrading plant and fungal cell wall polysaccharides. iScience, 26(4):106449(2023) [pubmed]

Keyword List

submitter keyword: bradysia
diptera
proteomics
saliva
glycosyl hydrolases
biomass

submitter keyword: bradysia

diptera

proteomics

saliva

glycosyl hydrolases

biomass

Contact List

Richard John Ward
contact affiliation	Departamento de Química, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto – SP, Brazil
contact email	rjward@fmrp.usp.br
lab head
Richard Ward
contact affiliation	Universidade de São Paulo
contact email	rjward@fmrp.usp.br
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/04/PXD033046
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/04/PXD033046

PRIDE project URI

Repository Record List

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