PXD033045-1

PXD033045 is an original dataset announced via ProteomeXchange.

Title	Nucleoporins facilitate ORC loading onto chromatin
Description	The origin recognition complex (ORC) binds throughout the genome to initiate DNA replication. In metazoans, it is still unclear how ORC is targeted to specific loci to facilitate helicase loading and replication initiation. Here, we performed immunoprecipitations coupled with mass spectrometry for ORC2 in Drosophila embryos. Surprisingly, we found that ORC2 associates with multiple subunits of the Nup107-160 subcomplex of the nuclear pore. Bioinformatic analysis revealed that, relative to all modENCODE factors, nucleoporins are among the most enriched factors at ORC2 binding sites. Critically, depletion of the nucleoporin Elys, a member of the Nup107-160 complex, results in decreased ORC2 loading onto chromatin. Depleting Elys also sensitized cells to replication fork stalling, which could reflect a defect in establishing dormant replication origins. Our work reveals a new connection between ORC, replication initiation and nucleoporins, highlighting a previously unrecognized function of nucleoporins in metazoan replication initiation.
HostingRepository	PRIDE
AnnounceDate	2022-10-19
AnnouncementXML	Submission_2022-10-19_09:51:46.941.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	LoganRichards
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-07 02:55:06	ID requested
⏵ 1	2022-10-19 09:51:47	announced

Dataset with its publication pending

submitter keyword: DNA replication, Drosophila, genome stability, Nuclear Pore

JaredNordman
contact affiliation	Department of Biological Sciences, Vanderbilt University
contact email	jared.nordman@vanderbilt.edu
lab head
LoganRichards
contact affiliation	Vanderbilt University
contact email	logan.r.richards@vanderbilt.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD033045
     1. Label: PRIDE project
     2. Name: Nucleoporins facilitate ORC loading onto chromatin