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PXD033039-2

PXD033039 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe CARD14WT/E138A interactome.
DescriptionPsoriasis is a chronic inflammatory disease of the skin for which current treatments are only partially effective. There is therefore an urgent need to develop novel therapies with higher efficacy through a better understanding of disease aetiology. Recently, it has been demonstrated that individuals with specific mutations in the gene encoding the adapter protein CARD14 have a high risk of developing psoriasis. Psoriasis associated CARD14 mutations result in enhanced activation of NF-kB transcription factors in keratinocytes. The CARD14/NF-kB axis may therefore be central in driving the pathogenesis of psoriasis. The aim of this project is to investigate the molecular mechanisms by which CARD14 activates the NF-kB pathway. Specifically, within this project, we aim to detail the interaction partners of both WT and a gain-of-function mutant CARD14 (E138A) via SILAC and mass spectrometry.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:56:43.434.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterHelen Flynn
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-04-07 02:45:07ID requested
12024-08-27 06:40:00announced
22024-10-22 06:56:44announced2024-10-22: Updated project metadata.
Publication List
O'Sullivan PA, Aidarova A, Afonina IS, Manils J, Thurston TLM, Instrell R, Howell M, Boeing S, Ranawana S, Herpels MB, Chetian R, Bassa M, Flynn H, Frith D, Snijders AP, Howes A, Beyaert R, Bowcock AM, Ley SC, CARD14 signalosome formation is associated with its endosomal relocation and mTORC1-induced keratinocyte proliferation. Biochem J, 481(18):1143-1171(2024) [pubmed]
10.1042/bcj20240058;
Keyword List
submitter keyword: CARMA, keratinocyte, MALT1, BCL10, psoriasis, NF-kappaB,CARD14
Contact List
Steven Ley
contact affiliationPears Chair of Molecular Immunology Institute of Immunity & Transplantation The Pears Building - Royal Free Hospital University College London
contact emailsteven.ley@ucl.ac.uk
lab head
Helen Flynn
contact affiliationThe Francis Crick Institute
contact emailhelen.flynn@crick.ac.uk
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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