PXD033036-1

PXD033036 is an original dataset announced via ProteomeXchange.

Title	Photo-ANA enables profiling of host–bacteria protein interactions during infection
Description	Bacterial pathogens rapidly change and adapt their proteome to cope with the environment in host cells and secret effector proteins to hijack host targets to ensure their survival and proliferation during infection. Excessive host proteins make it difficult to profile pathogens’ proteome dynamics by conventional proteomics. It is even more challenging to map pathogen-host protein-protein interactions in real time, given the low abundance of bacterial effectors and weak and transient interactions they may be involved. Here, we report a method for selectively labeling of bacterial proteome using a bifunctional amino acid, photo-ANA, equipped with a bioorthogonal handle and a photoreactive warhead, which enables simultaneous analysis of bacterial proteome reprogramming and pathogen-host protein interactions of Salmonella Typhimurium during infection. Using photo-ANA, we discovered temporal and distinct protein synthesis changes inside host cells and identified FLOT1/2 as the novel host interactors of Salmonella Typhimurium effector PipB2 in late stage of infection.
HostingRepository	PRIDE
AnnounceDate	2023-01-28
AnnouncementXML	Submission_2023-01-28_10:30:13.281.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	XiaomengLi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-07 02:43:57	ID requested
⏵ 1	2023-01-28 10:30:13	announced
2	2023-11-14 08:58:21	announced	2023-11-14: Updated project metadata.

submitter keyword: Salmonella

XIANG DAVIDLI
contact affiliation	The University of Hong Kong
contact email	xiangli@hku.hk
lab head
XiaomengLi
contact affiliation	The University of Hong Kong
contact email	lixm@hku.hk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD033036
     1. Label: PRIDE project
     2. Name: Photo-ANA enables profiling of host–bacteria protein interactions during infection