PXD033009 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The CARD14E138A phosphoproteome |
Description | Psoriasis is a chronic inflammatory disease of the skin for which current treatments are only partially effective. There is therefore an urgent need to develop novel therapies with higher efficacy through a better understanding of disease aetiology. Recently, it has been demonstrated that individuals with specific mutations in the gene encoding the adapter protein CARD14 have a high risk of developing psoriasis. Psoriasis associated CARD14 mutations result in enhanced activation of NF-kB transcription factors in keratinocytes. The CARD14/NF-kB axis may therefore be central in driving the pathogenesis of psoriasis. The aim of this project is to investigate the molecular mechanisms by which CARD14 activates the NF-kB pathway. Specifically, within this project, we aim to detail the downstream signalling pathways of a gain-of-function mutant CARD14 (E138A) via SILAC and mass spectrometry. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:56:44.772.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Helen Flynn |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-04-06 08:09:45 | ID requested | |
1 | 2024-08-27 07:45:43 | announced | |
⏵ 2 | 2024-10-22 06:56:45 | announced | 2024-10-22: Updated project metadata. |
Publication List
O'Sullivan PA, Aidarova A, Afonina IS, Manils J, Thurston TLM, Instrell R, Howell M, Boeing S, Ranawana S, Herpels MB, Chetian R, Bassa M, Flynn H, Frith D, Snijders AP, Howes A, Beyaert R, Bowcock AM, Ley SC, CARD14 signalosome formation is associated with its endosomal relocation and mTORC1-induced keratinocyte proliferation. Biochem J, 481(18):1143-1171(2024) [pubmed] |
10.1042/bcj20240058; |
Keyword List
submitter keyword: CARMA, keratinocyte, MALT1, BCL10, psoriasis, NF-kappaB,CARD14 |
Contact List
Steven Ley |
contact affiliation | Pears Chair of Molecular Immunology Institute of Immunity & Transplantation The Pears Building - Royal Free Hospital University College London |
contact email | steven.ley@ucl.ac.uk |
lab head | |
Helen Flynn |
contact affiliation | The Francis Crick Institute |
contact email | helen.flynn@crick.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/08/PXD033009 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD033009
- Label: PRIDE project
- Name: The CARD14E138A phosphoproteome