PXD033006-1

PXD033006 is an original dataset announced via ProteomeXchange.

Title	Placental macrophage (Hofbauer cell) response to viral and bacterial ligands is influenced by fetal sex
Description	Bacterial and viral infections of the placenta are associated with inflammation and adverse pregnancy outcomes. Hofbauer cells (HBCs) are specialised fetal-origin macrophages in the placental villi and are proposed to protect the fetus from vertical transmission of pathogens; however, they are poorly understood. Here, we have performed quantitative proteomics on term HBCs under resting conditions and following exposure to bacterial and viral pathogen associated molecular patterns (PAMPs), and investigated the contribution of fetal sex to these responses. Resting HBCs expressed a plethora of proteins pertinent to macrophage function, including chemokines, cytokines, Toll-like receptors, and classical and non-classical major histocompatibility complex class I and II molecules. HBCs mounted divergent responses to bacterial versus viral PAMPs but exhibited protein expression changes suggestive of a switch towards a more pro-inflammatory phenotype. A comparison between male and female HBCs, showed that the latter mounted a much stronger and wider response. Sexual dimorphism in HBCs was primarily associated with lipid metabolism in males and cytoskeleton organisation in females. We provide a novel and comprehensive understanding regarding the phenotype of term placental macrophages and their sex-dependent responses to infectious triggers.
HostingRepository	PRIDE
AnnounceDate	2022-10-31
AnnouncementXML	Submission_2022-10-31_05:29:46.201.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	PaschaliaPantazi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-06 08:04:46	ID requested
⏵ 1	2022-10-31 05:29:46	announced

Dataset with its publication pending

submitter keyword: placental macrophages, Hofbauer cells, nano LC-MSMS,human

BethHolder
contact affiliation	Dept of Metabolism Digestion and Reproduction, Imperial College London, London, UK
contact email	b.holder@imperial.ac.uk
lab head
PaschaliaPantazi
contact affiliation	Imperial College London
contact email	p.pantazi@imperial.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD033006
     1. Label: PRIDE project
     2. Name: Placental macrophage (Hofbauer cell) response to viral and bacterial ligands is influenced by fetal sex