PXD032991-1

PXD032991 is an original dataset announced via ProteomeXchange.

Title	Protein regulation mechanism of cold tolerance in Haemaphysalis longicornis
Description	BACKGROUND: Ticks are a kind of external parasitic arthropods that can transmit a variety of pathogens by sucking blood. Low temperature tolerance is an important guarantee for ticks to survive the cold winter. Exploring the protein regulation mechanism of low temperature tolerance of Haemaphysalis longicornis could help to explain how ticks survive in cold winter. At the same time, it has certain theoretical guiding significance for how to use winter to better control tick population. RESULTS: In this study, the quantitative proteomics of several tissues of Haemaphysalis longicornis exposed to low temperature was studied by DIA technology. 3699, 3422, and 1958 proteins were identified in salivary gland, midgut, and ovary, respectively. The proteins involved in energy metabolism, cell signal transduction, protein synthesis and repair, and cytoskeleton synthesis changed under low temperature stress. After a variety of bioinformatics analysis, it was found that arylamine N-acetyltransferase, inositol polyphosphate multikinase, and dual specificity phosphorase were of great significance in low temperature tolerance. Then we performed RNA interference on the mRNA of these three proteins, and the results showed that the ability of female ticks to tolerate low temperature decreased significantly. CONCLUSION: The comprehensive analysis of the protein regulation of multiple tissues of female ticks exposed to low temperature showed that maintaining cell homeostasis, maintaining cell viability, and enhancing cell tolerance were the most important means for ticks to maintain vital signs under low temperature. The expression of proteins involved in and regulating the above cell activities was the key to the survival of ticks under low temperature.
HostingRepository	iProX
AnnounceDate	2022-04-04
AnnouncementXML	Submission_2022-04-05_19:50:22.985.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ningmei Wang
SpeciesList	scientific name: Haemaphysalis longicornis; NCBI TaxID: 44386;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-05 19:50:02	ID requested
⏵ 1	2022-04-05 19:50:23	announced
2	2023-12-25 20:00:06	announced	2023-12-26: Update information.

Dataset with its publication pending

submitter keyword: Low temperature tolerance, Protein regulation, Tick, Survival strategy

Hui Wang
contact affiliation	Hebei Normal University
contact email	whui1981@163.com
lab head
Ningmei Wang
contact affiliation	Hebei Normal University
contact email	767247571@qq.com
dataset submitter

iProX dataset URI