PXD032961-1

PXD032961 is an original dataset announced via ProteomeXchange.

Title	The mechanisms of neutrophil dysfunction during sepsis
Description	The project examines the mechanisms of neutrophil dysfunction during sepsis. Our work uncovered the central role of cell free circulating histones in eliminating mature neutrophil in favour of immature cells and characterized the mechanisms that regulate their release following systemic infection. Mature and immature neutrophil Ly6Ghigh and Ly6Glow populations isolated from the spleens of WT and TCRα-deficient mice either naïve or infected with C. albicans were characterized. In addition, these populations were compared to neutrophils isolated from WT mice receiving Clodronate-liposomes and recombinant G-CSF. These studies demonstrated that T-cell derived histones drive the release of G-CSF in the spleen and progressively eliminate mature neutrophils by shortening their lifespan. Finally, we conducted proteomic analysis of plasmas isolated from patients with microbial sepsis to correlate markers of neutrophil death to plasma cytokine and histone levels, confirming the pathogenic role these molecules play during sepsis in humans.
HostingRepository	PRIDE
AnnounceDate	2022-12-13
AnnouncementXML	Submission_2022-12-13_10:01:40.901.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	SpyrosVernardis
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-03 07:24:27	ID requested
⏵ 1	2022-12-13 10:01:41	announced

Dataset with its publication pending

submitter keyword: mouse model,sepsis

MarkusRalser
contact affiliation	Einstein Professor of Biochemistry Head, Institute of Biochemistry, Charité Universitätsmedizin Berlin, Germany markus.ralser@charite.de Senior Group Leader, The Francis Crick Institute, London, UK markus.ralser@crick.ac.uk
contact email	markus.ralser@crick.ac.uk
lab head
SpyrosVernardis
contact affiliation	The Francis Crick Institute
contact email	spyros.vernardis@crick.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/12/PXD032961

PRIDE project URI

• PRIDE
  1. PXD032961
     1. Label: PRIDE project
     2. Name: The mechanisms of neutrophil dysfunction during sepsis