PXD032902-1

PXD032902 is an original dataset announced via ProteomeXchange.

Title	Combining recombinase-mediated cassette exchange strategy with quantitative proteomic and phosphoproteomic analyses to inspect intracellular functions of the tumor supressor galectin-4 in colorectal cancer cells.
Description	In order to systematically explore Galectin-4 function in CRC we established a CRC cell line where Gal4 expression can be regulated via doxycycline (dox)-inducible expression of a single copy wildtype LGALS4 transgene generated by recombinase-mediated cassette exchange (RMCE). Using this model and applying in depth proteomic and phosphoproteomic analyses we systematically screened for intracellular changes induced by Gal4 expression by.
HostingRepository	PRIDE
AnnounceDate	2022-08-12
AnnouncementXML	Submission_2022-08-12_00:10:45.581.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Malwina Michalak
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-02 10:31:56	ID requested
⏵ 1	2022-08-12 00:10:46	announced

Michalak M, Golde V, Helm D, Kaltner H, Gebert J, Kopitz J, Combining Recombinase-Mediated Cassette Exchange Strategy with Quantitative Proteomic and Phosphoproteomic Analyses to Inspect Intracellular Functions of the Tumor Suppressor Galectin-4 in Colorectal Cancer Cells. Int J Mol Sci, 23(12):(2022) [pubmed]

submitter keyword: Galectin, Colorectal Cancer

Juergen Kopitz
contact affiliation	Applied Tumor Biology, Institute of Pathology, Heidelberg University Hospital
contact email	Juergen.Kopitz@med.uni-heidelberg.de
lab head
Malwina Michalak
contact affiliation	Applied Tumor Biology, Institute of Pathology, Heidelberg University Hospital
contact email	malwina.michalak@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD032902
     1. Label: PRIDE project
     2. Name: Combining recombinase-mediated cassette exchange strategy with quantitative proteomic and phosphoproteomic analyses to inspect intracellular functions of the tumor supressor galectin-4 in colorectal cancer cells.