PXD032900-2

PXD032900 is an original dataset announced via ProteomeXchange.

Title	Impaired iron recycling from erythrocytes is an early hallmark of aging
Description	Both iron homeostasis and erythropoiesis are known to be affected by aging. Iron needs in mammals are met primarily by iron recycling from senescent red blood cells (RBCs), a task chiefly accomplished by red pulp macrophages (RPMs) in the spleen. Given that RPMs continuously process iron, their cellular functions might be susceptible to age-dependent decline, a possibility that has been unexplored to date. In our project, we identified a formation of undegradable iron- and heme-rich extracellular aggregates in the spleens of 10-11-month-old female mice. We further found that feeding mice an iron-reduced diet alleviates the deposition of iron in the spleen in the form of these insoluble aggregates. Here, we performed: i) label-free proteomic analysis of the aggregates that were magnetically isolated from the aged mice (maintained on a standard diet), using samples from young mice as a background control; and ii) TMT-based quantification of the differences in the aggregate protein composition between mice that aged on a standard versus iron-reduced diet. In each experiment, three biological replicates per group were analyzed.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:19:27.557.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Remigiusz Serwa
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-02 02:51:18	ID requested
1	2023-02-02 00:40:59	announced
⏵ 2	2023-11-14 07:19:28	announced	2023-11-14: Updated project metadata.

Slusarczyk P, Mandal PK, Zurawska G, Niklewicz M, Chouhan K, Mahadeva R, Jo, ń, czy A, Macias M, Szybinska A, Cybulska-Lubak M, Krawczyk O, Herman S, Mikula M, Serwa R, Lenartowicz M, Pokrzywa W, Mleczko-Sanecka K, Impaired iron recycling from erythrocytes is an early hallmark of aging. Elife, 12():(2023) [pubmed]

submitter keyword: ferritin,iron-recycling, ferroportin, proteostasis, hepcidin

Katarzyna Mleczko-Sanecka
contact affiliation	International Institute of Molecular and Cell Biology in Warsaw, Poland
contact email	kmsanecka@iimcb.gov.pl
lab head
Remigiusz Serwa
contact affiliation	The International Institute of Molecular Mechanisms and Machines, Polish Academy of Sciences
contact email	r.serwa@imol.edu.pl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD032900
     1. Label: PRIDE project
     2. Name: Impaired iron recycling from erythrocytes is an early hallmark of aging