PXD032844-1

PXD032844 is an original dataset announced via ProteomeXchange.

Title	Novel antibody-peptide binding assay indicates presence of immunoglobulins against EGFR phospho-site S1166 in high-grade glioma.
Description	The project’s aim was to develop and test the applicability of a method to determine the presence of serum immunoglobulins against specific autoantibodies. The method combined immunoglobulin G enrichment (using Melon Gel), molecular weight filtration, and targeted mass spectrometry and the feasibility was successfully tested in an experiment using a peptide library (tryptic digest) of dinitrophenyl labelled peptides and anti-DNP. In a second step, a phospho-peptide library, derived from glioblastoma multiform tissue, was tested against plasma samples from glioma patients and healthy donors, to determine the presence of complexes of IgG with immunoaffinity to EGFR or GFAP phospho-peptides. As a result, we found immunoaffinity to EGFR phospho-peptide GSHQIS[+80]LDNPDYQQDFFPK (covering the phospho-site S1166) in plasma of glioma patients.
HostingRepository	PRIDE
AnnounceDate	2024-10-07
AnnouncementXML	Submission_2024-10-07_13:45:58.878.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD032844
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Christoph Stingl
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; 2,4-dinitrophenyl modified residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-27 05:48:02	ID requested
⏵ 1	2024-10-07 13:45:59	announced

10.6019/PXD032844;

Zeneyedpour L, Stingl C, Kros JM, Sillevis Smitt PAE, Luider TM, Novel Antibody-Peptide Binding Assay Indicates Presence of Immunoglobulins against EGFR Phospho-Site S1166 in High-Grade Glioma. Int J Mol Sci, 23(9):(2022) [pubmed]

10.3390/ijms23095061;

submitter keyword: glioblastoma, Melon Gel, peptide antigen,autoantibodies, immunoglobulin G

Theo M. Luider
contact affiliation	Head of Laboratory of Neuro-Oncology, Neurology, Clinical & Cancer Proteomics
contact email	t.luider@erasmusmc.nl
lab head
Christoph Stingl
contact affiliation	Erasmus MC Rotterdam Department of Neurology
contact email	c.stingl@erasmusmc.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/06/PXD032844

PRIDE project URI

• PRIDE
  1. PXD032844
     1. Label: PRIDE project
     2. Name: Novel antibody-peptide binding assay indicates presence of immunoglobulins against EGFR phospho-site S1166 in high-grade glioma.