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PXD032792 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIntegrated Multi-Omics Reveal Polycomb Repressive Complex 2 Restricts Human Trophoblast Induction
DescriptionHuman naive pluripotent stem cells have unrestricted lineage potential. Underpinning this property, naive cells are thought to lack chromatin-based lineage barriers. However, this assumption has not been tested. Here, we define the chromatin-associated proteome, histone post-translational modifications and transcriptome of human naive and primed pluripotent stem cells. Our integrated analysis reveals differences in the relative abundance and activities of distinct chromatin modules. We identify a strong enrichment of Polycomb Repressive Complex 2 (PRC2)-associated H3K27me3 in naive pluripotent stem cell chromatin, and H3K27me3 enrichment at promoters of lineage-determining genes, including trophoblast regulators. PRC2 activity acts as a chromatin barrier restricting the differentiation of naive cells towards the trophoblast lineage, while inhibition of PRC2 promotes trophoblast fate induction and cavity formation in human blastoids. Together, our results establish that human naive pluripotent stem cells are not epigenetically unrestricted, but instead possess chromatin mechanisms that oppose the induction of alternative cell fates.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterNatarajan Bhanu
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonomethylated residue; acetylated residue
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-03-24 13:36:37ID requested
12022-05-09 02:41:34announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Polycomb Repressive Complex 2 (PRC2), human pluripotent stem cells, H3K27me3, trophoblast fate, chromatin proteomes
Contact List
Benjamin A Garcia
contact affiliationRaymond H. Wittcoff Distinguished Professor and Head Department of Biochemistry and Molecular Biophysics Washington University School of Medicine St. Louis, MO 63110
contact emailbagarcia@wustl.edu
lab head
Natarajan Bhanu
contact affiliationWashington University
contact emailbhanujayavel@hotmail.com
dataset submitter
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