PXD032748-2

PXD032748 is an original dataset announced via ProteomeXchange.

Title	Comparative proteomic of Trypanosoma cruzi strains
Description	Trypanosoma cruzi, the causative agent of Chagas disease, is a genetically diverse with TcI being the most widespread lineage in the Americas. The protein expression dynamics allows unravel the biological phenomena of pathogens such as disease development and evolution. A large number of studies have shown a direct relationship between genetic variability and the biological characteristics of T. cruzi. However, much remains to be explored regarding the impact of gene expression on the development and evolution of Chagas disease. Two-dimensional (2D) electrophoresis and mass-spectrometry were employed to characterize the global protein expression patterns of epimastigotes from four distinct TcI strains, which display different growth kinetics. Biological differences were identified and confirmed throug metabolic and morphometric assays. Statistical tests (parametric or non-parametric test, as appropriate for the experiment) were used to assess the significance of the observed differences. The Ascending hierarchical classification analysis shows two clusters, that were congruent with their growth kinetics. The proteins expressed in one or the other cluster were identified by mass spectrometry. These proteins were evaluated by independent biological assays, which showed that the parasite’s metabolic activity and morphology is significantly different between the two clusters. We conclude that the protein expression patterns displayed by epimastigotes from fast/slow growth TcI strains are correlated with various biological parameters, including intensity of metabolic activity, metabolic fuel usage and resistance to oxidative stress, as well as morphological characteristics, such as length of the flagellum.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:02:02.194.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Functional Proteomics Platform FPP
SpeciesList	scientific name: Trypanosoma cruzi; NCBI TaxID: 5693;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap XL

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-23 07:35:03	ID requested
1	2023-07-12 02:28:05	announced
⏵ 2	2023-11-14 09:02:13	announced	2023-11-14: Updated project metadata.

Telleria J, Tibayrenc M, Del Salto Mendoza M, Seveno M, Costales JA, Comparative proteomic analysis of Trypanosoma cruzi TcI lineage epimastigotes unveils metabolic and phenotypic differences between fast- and slow-dividing strains. Exp Parasitol, 252():108576(2023) [pubmed]

submitter keyword: growth kinetics,Chagas disease

Jaime Alfredo Costales Cordero
contact affiliation	Centro de Investigación para la Salud en América Latina, Pontificia Universidad Católica del Ecuador, Quito, Ecuador
contact email	JACOSTALESC@puce.edu.ec
lab head
Functional Proteomics Platform FPP
contact affiliation	FPP CNRS
contact email	contact@fpp.cnrs.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/07/PXD032748

PRIDE project URI

• PRIDE
  1. PXD032748
     1. Label: PRIDE project
     2. Name: Comparative proteomic of Trypanosoma cruzi strains