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PXD032736-1

PXD032736 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleApplication of Two-Dimensional Difference Gel Electrophoresis to Identify Protein Changes between Center, Margin, and Adjacent Non-Tumor Tissues Obtained from Non-Small-Cell Lung Cancer with Adenocarcinoma Subtype
DescriptionLung cancer is responsible for the most cancer-related mortality worldwide and the mechanism of its development is poorly understood. Proteomics has become a powerful tool offering vital knowledge related to cancer development. Using a two-dimensional difference gel electrophoresis (2D-DIGE) approach, we sought to compare tissue samples from non-small-cell lung cancer (NSCLC) patients, exactly adenocarcinoma (ADC), taken from the tumor center and tumor margin and control (adjacent non-tumor) tissues . We found 22 differentially abundant spots representing 17 proteins differentiating center and margin. Sixty eight proteins; represented by 79 spots :40, 20, and 19 spots differed between the control and center and margin, control and center, and control and margin, respectively. Twenty six significant canonical pathways were identified, including Rho signaling pathways, a semaphorin neuronal repulsive signaling pathway, and epithelial adherens junction signaling. Proteins differentiating the tumor center and tumor margin were linked to cancer invasion and progression, including cell migration, adhesion and invasion, cytoskeletal structure, protein folding, anaerobic metabolism, tumor angiogenesis, EMC transition, epithelial adherens junctions, and inflammatory responses.
HostingRepositoryPRIDE
AnnounceDate2024-10-08
AnnouncementXMLSubmission_2024-10-08_11:39:40.507.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMariola Dietrich
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
Instrumentautoflex TOF/TOF; autoflex
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-03-23 07:23:52ID requested
12024-10-08 11:39:45announced
Publication List
10.1371/journal.pone.0268073;
Ciereszko A, Dietrich MA, S, ł, owi, ń, ska M, Nynca J, Ciborowski M, Kaczmarek MM, Myszczy, ń, ski K, Ki, ś, luk J, Majewska A, Michalska-Falkowska A, Kodzik N, Resze, ć J, Sierko E, Nikli, ń, ski J, Application of two-dimensional difference gel electrophoresis to identify protein changes between center, margin, and adjacent non-tumor tissues obtained from non-small-cell lung cancer with adenocarcinoma or squamous cell carcinoma subtype. PLoS One, 17(5):e0268073(2022) [pubmed]
Keyword List
submitter keyword: cancer, lung tissue,human, 2D-DIGE, adenocarcinoma
Contact List
Mariola Dietrich
contact affiliationDepartment of Gametes and Embryo Biology, Institute of Animal Reproduction and Food Research Polish Academy of Sciences, Olsztyn, 10-748, Poland
contact emailm.dietrich@pan.olsztyn.pl
lab head
Mariola Dietrich
contact affiliationPolish Academy of Sciences
contact emailm.dietrich@pan.olsztyn.pl
dataset submitter
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Dataset FTP location
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