PXD032722-1

PXD032722 is an original dataset announced via ProteomeXchange.

Title	Comparative proteomics analysis of FFPE testicular tissue from men with obstructive and non-obstructive azoospermia
Description	The project aimed to compare testicular proteomes from patients with obstructive and non-obstructive azoospermia in order to identify molecular signatures involved in spermatogenesis as well to identify candidate biomarkers for discriminating between different types of azoospermia. The samples used in this study were formalin fixed paraffin embedded (FFPE) testicular tissues obtained by biopsy from men with clinical diagnosis of azoospermia. Samples were grouped according to the histopathological report in 3 groups: 1) Spermatogenesis (obstructive azoospermia), 2) Hypospermatogenesis and 3) Sertoli cell only syndrome (SCO). Patients were aged 31–46 years with no differences among groups regarding age (Median age (Hypospermatogenesis) =35; Median age (SCO) =34 and Median age (Spermatogenesis) =39). Each group had 9 samples or 27 samples in total were used for the comparative proteomics analysis by label-free data-independent LC-MS/MS.
HostingRepository	PRIDE
AnnounceDate	2022-07-31
AnnouncementXML	Submission_2022-07-31_12:57:50.059.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD032722
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Katarina Davalieva
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-23 06:09:28	ID requested
⏵ 1	2022-07-31 12:57:50	announced
2	2023-11-14 08:55:53	announced	2023-11-14: Updated project metadata.
3	2024-10-22 05:38:27	announced	2024-10-22: Updated project metadata.

10.1016/J.JPROT.2022.104686;

submitter keyword: Human, testis, FFPE, Azoospermia, Rapigest, LC-MS/MS

Katarina Davalieva
contact affiliation	Research Centre for Genetic Engineering and Biotechnology “Georgi D Efremov”, Macedonian Academy of Sciences and Arts, Skopje, Krste Misirkov 2, 1000 Skopje, Republic of North Macedonia
contact email	katarina@manu.edu.mk
lab head
Katarina Davalieva
contact affiliation	Research Centre for Genetic Engineering and Biotechnology “Georgi D Efremov”, Macedonian Academy of Sciences and Arts, Skopje
contact email	katarina@manu.edu.mk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/07/PXD032722

PRIDE project URI

• PRIDE
  1. PXD032722
     1. Label: PRIDE project
     2. Name: Comparative proteomics analysis of FFPE testicular tissue from men with obstructive and non-obstructive azoospermia