PXD032703-1
PXD032703 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Characterization of oligopeptides in Solanum lycopersicum xylem exudates |
| Description | We collected 120 ml of xylem exudate from the cut surface of the first internodes from the hypocotyls of the tomato plants at 45 DAG for five hours. The exudates were purified using the gel-free method that we developed previously (Okamoto et al., 2015, Plant J.). Using gel filtration chromatography (Superdex Peptide 10/300 GL gel filtration column (10 mm 300 mm; GE Healthcare)), we separated small-protein-containing fractions (small-protein fractions) and oligopeptide-containing fractions (peptide fractions). The small-protein fractions were treated with trypsin, and the peptide fractions were further purified with multimode chromatography (Asahipak GS-320 column (7.5 mm 300 mm; Shodex)). Then, data-dependent MS/MS (ddMS/MS) was performed using an EASY-nLC 1000 (Thermo Fisher Scientific) connected to an Orbitrap Elite mass spectrometer (Thermo Fisher Scientific). |
| HostingRepository | jPOST |
| AnnounceDate | 2022-04-15 |
| AnnouncementXML | Submission_2022-04-14_23:10:08.000.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Satoru Okamoto |
| SpeciesList | scientific name: cellular organisms; NCBI TaxID: 131567; |
| ModificationList | unknown modification; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine; monohydroxylated proline; S-carboxamidomethyl-L-cysteine; monohydroxylated proline |
| Instrument | LTQ Orbitrap |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2022-03-22 01:34:09 | ID requested | |
| ⏵ 1 | 2022-04-14 23:10:08 | announced | |
| 2 | 2022-09-18 02:35:09 | announced | 2022-09-18: Updated FTP location. |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Solanum lycopersicum, peptide, xylem sap |
Contact List
| Satoru Okamoto | |
|---|---|
| lab head | |
| Satoru Okamoto | |
| contact affiliation | Niigata University |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.biosciencedbc.jp/archive/jpostrepos/JPST001538 |
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