PXD032394-2

PXD032394 is an original dataset announced via ProteomeXchange.

Title	Comparison of performances of automatic versus manual liquid handling in density-based separation of extracellular vesicles from plasma and urine
Description	Extracellular vesicles (EV) in body fluids are extensively studied as potential biomarkers for numerous diseases. Major impediments of EV-based biomarker discovery include the manual labor, and the specificity and reproducibility of EV sample preparation. To tackle this, we present an automated liquid handling workstation for the density-based separation of EV from human body fluids and compare its performance to manual handling by (in)experienced researchers. To validate automated density-based separation of EVs from human body fluids, including blood plasma and urine, we assess variation, yield and purity by mass spectrometry-based proteomics.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:55:24.954.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pekka Rappu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-18 09:01:21	ID requested
1	2023-05-09 23:38:49	announced
⏵ 2	2023-11-14 08:55:25	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: Plasma, urine, extracellular vesicle, automatic liquid handling

Olivier De Wever
contact affiliation	Laboratory of Experimental Cancer Research, Department of Human Structure and Repair, Ghent University, Ghent, Belgium
contact email	Olivier.DeWever@UGent.be
lab head
Pekka Rappu
contact affiliation	University of Turku
contact email	pekka.rappu@utu.fi
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD032394

PRIDE project URI

• PRIDE
  1. PXD032394
     1. Label: PRIDE project
     2. Name: Comparison of performances of automatic versus manual liquid handling in density-based separation of extracellular vesicles from plasma and urine