PXD032374-1

PXD032374 is an original dataset announced via ProteomeXchange.

Title	Rapid and specific degradation of endogenous proteins in mouse models using auxin-inducible degrons
Description	Auxin-inducible degrons are a chemical genetic tool for targeted protein degradation and are widely used to study protein function in cultured mammalian cells. Here, we develop CRISPR-engineered mouse lines that enable rapid and highly specific degradation of tagged endogenous proteins in vivo. Most but not all cell types are competent for degradation. Using mouse genetics, we show that degradation kinetics depend upon the dose of the tagged protein, ligand, and the E3 ligase subunit Tir1. Rapid degradation of condensin I and condensin II – two essential regulators of mitotic chromosome structure - revealed that both complexes are individually required for cell division in precursor lymphocytes, but not in their differentiated peripheral lymphocyte derivatives. This generalisable approach provides unprecedented temporal control over the dose of endogenous proteins in mouse models, with implications for studying essential biological pathways and modelling drug activity in mammalian tissues.
HostingRepository	PRIDE
AnnounceDate	2022-06-07
AnnouncementXML	Submission_2022-06-07_03:45:51.003.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alex von kriegsheim
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-17 09:42:19	ID requested
⏵ 1	2022-06-07 03:45:51	announced
2	2024-10-22 05:37:31	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Auxin degradation, DIA, Condensin

Andrew Wood
contact affiliation	University of Edinburgh
contact email	a.wood@ed.ac.uk
lab head
Alex von kriegsheim
contact affiliation	University of Edinburgh
contact email	alex.vonkriegsheim@ucd.ie
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/06/PXD032374

PRIDE project URI

• PRIDE
  1. PXD032374
     1. Label: PRIDE project
     2. Name: Rapid and specific degradation of endogenous proteins in mouse models using auxin-inducible degrons