PXD032356-1

PXD032356 is an original dataset announced via ProteomeXchange.

Title	FLEXIBLE AND DIGESTIBLE WOOD CAUSED BY VIRAL-INDUCED ALTERATION OF CELL WALL COMPOSITION
Description	Woody plant material represents a vast renewable resource that has the potential to produce biofuels and other bio-based products with favourable net CO2 emissions1,2. Its potential has been demonstrated in a recent study that generated novel structural materials from flexible mouldable wood3. The study of viral infection in plants has largely focussed on detrimental symptoms, such as leaf yellowing or cell death that result in reduced crop yields. Apple rubbery wood (ARW) disease is the result of a viral infection that causes woody stems to exhibit increased flexibility4. While ARW disease is associated with the presence of an RNA virus5 (ARWV), the mechanism underlying the development of its unique symptoms is not known. We demonstrate that ARWV symptoms arise from reduced lignification within the secondary cell wall of xylem fibres, while the mid-lamellae region and xylem ray cells are largely unaffected, and results in increased wood digestibility. Gene expression and proteomic data from symptomatic xylem clearly show the downregulation of phenylalanine ammonia lyase (PAL), the enzyme catalysing the first committed step in the phenylpropanoid pathway leading 2 to lignin biosynthesis. PAL downregulation is the likely cause of the decreased lignification, which is consistent with a large increase in soluble phenolics, including the lignin precursor phenylalanine, seen in symptomatic xylem. ARWV-infection results in the accumulation of many host-derived viral activated small interfering RNAs (vasiRNAs). PAL-derived vasiRNAs are among the most abundant vasiRNAs in symptomatic xylem and are likely the cause of reduced PAL activity. Apparently, the mechanism used by the virus to alter lignin exhibits similarities to the RNAi strategy that has been used to alter lignin in genetically modified trees to generate comparable improvements in wood properties6–8. CRISPR technology was recently used to increase yields in maize by introducing the naturally occurring waxy mutation into maize9. A similar biomimetic approach for biomass improvement based upon our understanding of ARWV symptoms offers a promising route to improve wood properties and provides important context for the genetic manipulation of trees.
HostingRepository	PRIDE
AnnounceDate	2022-06-26
AnnouncementXML	Submission_2022-06-26_04:54:50.790.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD032356
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Manoj Kumar
SpeciesList	scientific name: Malus domestica; NCBI TaxID: 3750;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-17 04:19:35	ID requested
⏵ 1	2022-06-26 04:54:51	announced

Dataset with its publication pending

submitter keyword: Plant Cell Walls, Apple Rubbery Wood, Metobolomics, Lignin, Cellulose

Simon Turner
contact affiliation	Faculty of Biology, Medicine and Health University of Manchester
contact email	simon.turner@manchester.ac.uk
lab head
Manoj Kumar
contact affiliation	University of Manchester
contact email	manoj.kumar@manchester.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/06/PXD032356

PRIDE project URI

• PRIDE
  1. PXD032356
     1. Label: PRIDE project
     2. Name: FLEXIBLE AND DIGESTIBLE WOOD CAUSED BY VIRAL-INDUCED ALTERATION OF CELL WALL COMPOSITION