PXD032346-2

PXD032346 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Identification of CLPX binding proteins in FT293 cells
Description	FT293 cells which express FLAG-tagged CLPX, in which deleted endogenous CLPX were used for the study. FT 293 cells which express a chimeric protein composed of the mitochondrial transit peptide region of ornithine transcarbamylase and Flag-tagged luciferase were used as control. Each of FLAG-tagged proteins were expressed by doxycycline-inducible manner. The FLAG-tagged proteins were immunoprecipitated using anti-DDDK-tag antibody-conjugated agarose beads, and the immunoprecipitated proteins were eluted from the agarose beads by DDDDK peptides. The eluted precipitates were digested with trypsin.
HostingRepository	jPOST
AnnounceDate	2022-03-17
AnnouncementXML	Submission_2022-09-18_03:52:11.001.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ko Suzuki
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide
Instrument	LTQ Orbitrap

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-17 01:48:00	ID requested
1	2022-03-17 01:48:02	announced
⏵ 2	2022-09-18 03:52:11	announced	2022-09-18: Updated FTP location.
3	2023-09-03 22:11:06	announced	2023-09-04: Updated PubMed.

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: FT293 cells, FLAG-IP

submitter keyword: FT293 cells, FLAG-IP

Contact List

Ko Suzuki
lab head
Ko Suzuki
contact affiliation	Iwate Medical University
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001532/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001532/