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PXD032328-1

PXD032328 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTMEM260 is a protein-specific O-mannosyltransferase
DescriptionMutations in the TMEM260 gene cause structural heart defects and renal anomalies syndrome (SHDRA), but the function of the encoded protein remains unknown. We report that TMEM260 is an ER-located protein O-mannosyltransferase that selectively glycosylates defined extracellular immunoglobulin, plexin, transcription factor (IPT) domains of the hepatocyte growth factor receptor (cMET), macrophage-stimulating protein receptor (RON), and plexin receptors. We demonstrate that disease-causing TMEM260 mutations impair O-mannosylation of IPT domains and that TMEM260 knock out in cells results in receptor maturation defects and abnormal growth of 3D cell models. Thus, our study identifies a new, receptor-specific O-mannosylation pathway that serves critical functions during e.g. epithelial morphogenesis.
HostingRepositoryPRIDE
AnnounceDate2023-05-05
AnnouncementXMLSubmission_2023-05-05_06:07:21.137.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAdnanHalim
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; hexosylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos; Orbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-03-16 09:10:53ID requested
12023-05-05 06:07:21announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: O-mannosylation, glycoproteomics, glycopeptide,TMEM260
Contact List
AdnanHalim
contact affiliationCopenhagen Center for Glycomics (CCG), Department of Cellular and Molecular Medicine, University of Copenhagen, Denmark
contact emailhalim@sund.ku.dk
lab head
AdnanHalim
contact affiliationUniversity of Copenhagen
contact emailhalim@sund.ku.dk
dataset submitter
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Dataset FTP location
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