PXD032322-1

PXD032322 is an original dataset announced via ProteomeXchange.

Title	Proteomic and functional analysis of the effects of quinoxaline derivatives on Entamoeba histolytica
Description	Quinoxalines are heterocyclic compounds that contain a benzene ring and a pyrazine ring. The oxidation of both nitrogen of the pyrazine ring results in quinoxaline derivatives (QdNO), which exhibit a variety of biological properties, including antiparasitic activity. However, its activity against Entamoeba histolytica, the protozoan that causes human amebiasis, is poorly understood. Recently, our group reported that various QdNOs produce morphological changes in E. histolytica trophozoites, increase reactive oxygen species, and inhibit thioredoxin reductase activity. Notably, T-001 and T-017 derivatives were among the QdNOs with the best activity. In order to contribute to the characterization of the antiamebic effect of QdNOs, in this work we analyzed the proteomic profile of E. histolytica trophozoites treated with the QdNOs T-001 and T-017, and the results were correlated with functional assays. A total number of 163 deregulated proteins were found in trophozoites treated with T-001, and 131 in those treated with T-017. A set of 21 overexpressed and 24 under-expressed proteins was identified, which were mainly related to cytoskeleton and intracellular traffic, nucleic acid transcription, translation and binding, and redox homeostasis. Furthermore, T-001 and T-017 modified the virulence of trophozoites, since they altered their erythrophagocytosis, migration, adhesion and cytolytic capacity. Our results show that in addition to alter reactive oxygen species, and thioredoxin reductase activity, T-001 and T-017 affect essential functions related to the actin cytoskeleton, which eventually affects E. histolytica virulence and survival.
HostingRepository	PRIDE
AnnounceDate	2022-07-04
AnnouncementXML	Submission_2022-07-04_09:50:02.840.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Oscar Medina Contreras
SpeciesList	scientific name: Entamoeba histolytica HM-1:IMSS; NCBI TaxID: 294381;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	ultrafleXtreme

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-16 04:14:15	ID requested
⏵ 1	2022-07-04 09:50:03	announced
2	2023-11-14 08:55:56	announced	2023-11-14: Updated project metadata.

10.3389/FCIMB.2022.887647;

submitter keyword: E. histolytica, quinoxaline derivatives, Proteomics, Functional Analysis, Antiamoebic effect

Maria Esther Ramirez Moreno
contact affiliation	Instituto Politecnico Nacional, Escuela Nacional de Medicina y Homeopatia, Laboratorio de Biomedicina Molecular, Mexico City, Mexico
contact email	maramirezmo@ipn.mx
lab head
Oscar Medina Contreras
contact affiliation	Mexico Children's Hospital
contact email	omedina@himfg.edu.mx
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD032322
     1. Label: PRIDE project
     2. Name: Proteomic and functional analysis of the effects of quinoxaline derivatives on Entamoeba histolytica