PXD032289-1
PXD032289 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Comprehensive Analysis of the Relationships between the Gut Microbiota and Fecal Metabolome in Individuals with Primary Sjogren’s Syndrome by 16S rRNA Sequencing and LC–MS-Based Metabolomics |
Description | The gut microbiota has been associated with primary Sjogren’s syndrome (pSS), yet the biological implications of these associations are often elusive. We characterized the fecal microbiota (16S rRNA gene amplification and sequencing) and the fecal metabolome (ultrahigh-performance liquid chromatography–mass spectrometry) in 30 patients with pSS and 20 healthy controls (HCs). In addition, microbial and metabolic data were cross-correlated to identify meaningful associations. We found that the microbiota composition of pSS patients was significantly different from that of HCs. The pSS gut microbiota is characterized by increased abundances of proinflammatory microbes, especially Escherichia-Shigella, and decreased abundances of anti-inflammatory microbes. Concerning the metabolome, a multivariate model with 33 metabolites efficiently distinguished cases from controls. Through KEGG enrichment analysis, we found that these metabolites were mainly involved in amino acid metabolism and lipid metabolism. The correlation analysis indicated that there were significant correlations between the microbiota and metabolism in pSS patients. In addition, an abundance of Escherichia-Shigella was found to be correlated with high levels of four metabolites (aflatoxin M1, glycocholic acid, L-histidine and phenylglyoxylic acid). Our research suggests that in pSS, the gut microbiota is characterized by a specific combination of proinflammatory changes and metabolic states. Escherichia-Shigella is a factor related to gut dysbiosis, which may promote intestinal damage and affect amino acid metabolism. |
HostingRepository | iProX |
AnnounceDate | 2022-03-10 |
AnnouncementXML | Submission_2022-03-14_22:21:25.626.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Yang Li |
SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2022-03-14 22:21:14 | ID requested | |
⏵ 1 | 2022-03-14 22:21:25 | announced | |
2 | 2022-09-29 23:40:03 | announced | 2022-09-30: Update publication information. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: primary Sjogren’s Syndrome, gut microbiota, 16S rRNA sequencing, metabolome, LC–MS, Escherichia-Shigella |
Contact List
Yang Li | |
---|---|
contact affiliation | Mianyang Central Hospital |
contact email | shiyuzhu55@163.com |
lab head | |
Yang Li | |
contact affiliation | Mianyang Central Hospital |
contact email | shiyuzhu55@163.com |
dataset submitter |
Full Dataset Link List
iProX dataset URI |