PXD032270-1

PXD032270 is an original dataset announced via ProteomeXchange.

Title	The role of testosterone in spermatogenesis: lessons from proteome profiling of human spermatozoa in testosterone deficiency
Description	Testosterone is essential to maintain qualitative spermatogenesis. Nonetheless, no studies have been yet performed in humans to analyze the testosterone-mediated expression of the sperm proteins and their importance in reproduction. Thus, this study aimed to identify sperm protein alterations in male hypogonadism using proteomic profiling. We have performed a comparative proteomic analysis comparing sperm from fertile controls (a pool of 5 normogonadic normozoospermic fertile men) versus sperm from patients with secondary hypogonadism (a pool of 5 oligozoospermic hypogonadic patients due to isolated LH deficiency). Sperm protein composition was analyzed, after peptide labelling with Isobaric Tags, via liquid chromatography followed by tandem mass spectrometry (LC-MS/MS) on an LTQ Velos-Orbitrap mass spectrometer. LC-MS/MS data were analyzed using Proteome Discoverer. Criteria used to accept protein identification included a false discovery rate (FDR) of 1% and at least 1 peptide match per protein. Up to 986 proteins were identified and, of those, 43 proteins were differentially expressed: 32 proteins were under-expressed and 11 were over-expressed in the pool of hypogonadic patients. Bioinformatic analyses were performed using UniProt Knowledgebase, and the Gene Ontology Consortium database based on PANTHER. Notably, 13 of these 43 differentially expressed proteins have been previously reported to be related to sperm function and spermatogenesis. Western blot analyses for A-Kinase Anchoring Protein 3 (AKAP3) and the Prolactin Inducible Protein (PIP) were used to confirm the proteomics data. In summary, a high-resolution mass spectrometry-based proteomic approach was used for the first time to describe alterations of the sperm proteome in secondary male hypogonadism. A panel of deregulated sperm proteins was identified, including Prosaposin, Ropporin-1B, SERPINA5, SMOC-1, SPANXB1, GSG1, ELSPBP1, fibronectin, 5-oxoprolinase, AKAP3, AKAP4, HYDIN, ROPN1B, ß-Microseminoprotein and Protein S100-A8, which might represent putative physiological in vivo targets for androgen deficiency.
HostingRepository	PRIDE
AnnounceDate	2022-05-09
AnnouncementXML	Submission_2022-05-09_04:09:14.433.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rafael Oliva
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-14 09:48:24	ID requested
⏵ 1	2022-05-09 04:09:14	announced

Dataset with its publication pending

submitter keyword: Male infertility, sperm, proteomics, TMT, hypogonadism, LH deficiency, testosterone

Rafael Oliva Virgili
contact affiliation	Genetics Unit, Department of Biomedical Sciences, Faculty of Medicine, Casanova 143, 08036 Barcelona, Spain
contact email	roliva@ub.edu
lab head
Rafael Oliva
contact affiliation	Director Molecular Biology of Reproduction and Development research Group, Genetics Unit, Faculty of Medicine, University of Barcelona, Institut d'Investigacions Biomèdiques August Pi iSunyer (IDIBAPS), and Hospital Clinic, Barcelona, Spain.
contact email	roliva@ub.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD032270
     1. Label: PRIDE project
     2. Name: The role of testosterone in spermatogenesis: lessons from proteome profiling of human spermatozoa in testosterone deficiency