PXD032203 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Detection of Cell-Cell Interactions via Photocatalytic Cell Tagging |
Description | The growing appreciation of immune cell-cell interactions within disease environments has led to significant efforts to develop highly effective protein- and cell-based immunotherapies. However, characterizing these complex cell-cell interfaces in high resolution remains challenging. Thus, technologies that can leverage therapeutic-based modalities to profile intercellular environments offer the opportunity to study cell-cell interactions with molecular-level insight. To address this, we introduce Photocatalytic Cell Tagging (PhoTag), a platform for profiling cell-cell interactions utilizing a single domain antibody (VHH) conjugated to a photoactivatable flavin-based cofactor. Upon irradiation with visible light, the tethered flavin photocatalyst generates phenoxy radical tags for targeted labeling within cell-cell contact regions. Using anti-PD-1 or anti-PD-L1 VHH flavin conjugates, we demonstrate that PhoTag achieves highly selective synaptic labeling in antigen presenting cell-T cell co-culture systems. By combining the high resolution transcellular biotinylation capability of PhoTag with multi-omics single cell sequencing, we interrogated transient interactions between peripheral blood mononuclear cell (PBMC) populations and Raji PD-L1 B cells and discovered that specific T cell subtypes can transiently interact more efficiently than others. We envision that the spatiotemporal and modular nature of PhoTag will enable its broad utilization for detailed profiling of intercellular interactions across different biological systems. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:55:20.558.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD032203 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Rob Oslund |
SpeciesList | scientific name: Oryctolagus cuniculus (Rabbit); NCBI TaxID: 9986; scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; scientific name: Gallus gallus (Chicken); NCBI TaxID: 9031; |
ModificationList | monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-03-11 09:23:28 | ID requested | |
1 | 2022-06-03 05:21:10 | announced | |
⏵ 2 | 2023-11-14 08:55:21 | announced | 2023-11-14: Updated project metadata. |
Publication List
Keyword List
submitter keyword: Flavin-based photocatalysis, phenoxy radical generation, tyrosine labeling |
Contact List
Rob Oslund |
contact affiliation | PhD |
contact email | rob.oslund@gmail.com |
lab head | |
Rob Oslund |
contact affiliation | Merck Exploratory Science Center |
contact email | rob.oslund@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD032203
- Label: PRIDE project
- Name: Detection of Cell-Cell Interactions via Photocatalytic Cell Tagging